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| Status |
Public on Dec 28, 2006 |
| Title |
Vibrio cholerae stringent response |
| Organism |
Vibrio cholerae |
| Experiment type |
Expression profiling by array
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| Summary |
This study is an analysis of changes in gene expression during stringent response in Vibrio cholerae. V. cholerae cells in mid-log were treated with serine hydroxamate and gene expression was compared to untreated cells.
Keywords: Stress response, stringent response
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| Overall design |
V. cholerae cells in mid-log were treated with the stringent response inducer serine hydroxamate for one doubling time, then RNA was harvested using a Trizol method. Mock-treated cells (V. cholerae with dH2O added) also had RNA harvested. Both serine-hydroxamate treated and wild type cell RNA was converted to cDNA, with one set incorporating Cy5, the other Cy3, with fluorophores reversed in the multiple analyses. RNA from both conditions was applied to a whole genome PCR microarray from V. cholerae N16961, containing two sets of every ORF. Data was analyzed by Genepix and Rosetta Resolver.
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| Citation(s) |
17360576 |
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| Submission date |
Dec 28, 2006 |
| Last update date |
Mar 17, 2012 |
| Contact name |
David Raskin |
| E-mail(s) |
draskin@hms.harvard.edu
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| Phone |
(617)432-2355
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| Fax |
(617)738-7664
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| Organization name |
Harvard Medical School
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| Department |
Microbiology & Molecular Genetics
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| Street address |
200 Longwood Avenue
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE6618 |
Regulation of the stringent response is the essential function of the conserved bacterial G protein CgtA in V. cholerae |
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| Relations |
| BioProject |
PRJNA104181 |
