GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE66049 Query DataSets for GSE66049
Status Public on Jan 07, 2016
Title Maternal DNA methylation regulates early trophoblast development
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary Critical roles for DNA methylation in embryonic development are well established, but less is known about the roles of DNA methylation during trophoblast development, the extraembryonic lineage that gives rise to the placenta. Here we dissected the role of DNA methylation in trophoblast development by performing mRNA and DNA methylation profiling of Dnmt3a/3b-null trophoblast. We find that most gene deregulation is explained by an erasure of maternal methylation in the oocyte, but partially independent of loss of imprinting of the trophoblast-essential Ascl2 gene. Our results reveal that maternal DNA methylation controls multiple differentiation and physiological processes in trophoblast via both imprinting-dependent and -independent mechanisms.
Overall design mRNA-seq and WGBS-seq of maternal Dnmt3a/3b-null trophoblast; mRNA-seq of maternal Ascl2 KO trophoblast
Contributor(s) Branco M, King M, Bogutz A, Lefebvre L, Cook SJ, Dean W, Hemberger M, Reik W
Citation(s) 26812015
Submission date Feb 18, 2015
Last update date May 15, 2019
Contact name Felix Krueger
Organization name The Babraham Institute
Department Bioinformatics
Street address Babraham Hall
City Cambridge
ZIP/Postal code CB22 3AT
Country United Kingdom
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (29)
GSM1613537 E7.5_EPC_Ctrl-1 RNA-Seq
GSM1613538 E7.5_EPC_Ctrl-2 RNA-Seq
GSM1613539 E7.5_EPC_3bKO-1 RNA-Seq
BioProject PRJNA275780
SRA SRP055147

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66049_Ascl2KO_expression_vsd.txt.gz 606.8 Kb (ftp)(http) TXT
GSE66049_Dnmt3KO_expression_vsd.txt.gz 1.4 Mb (ftp)(http) TXT
GSE66049_RAW.tar 809.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap