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Series GSE6595 Query DataSets for GSE6595
Status Public on Jan 01, 2007
Title Gene expression study in 1 and 2 cell cloned mice embryos
Organism Mus musculus
Experiment type Expression profiling by array
Summary Cloned embryos produced by somatic cell nuclear transfer (SCNT) display a plethora of phenotypic characteristics that make them different from fertilized embryos, indicating defects in the process of nuclear reprogramming by the recipient ooplasm. To elucidate the extent and timing of nuclear reprogramming, we used microarrays to analyze the transcriptome of mouse SCNT embryos during the first two cell cycles. We identified a large number of genes mis-expressed in SCNT embryos. We found that genes involved in transcription and regulation of transcription are prominent among affected genes, and thus may be particularly difficult to reprogram, and these likely cause a ripple effect that alters the transcriptome of many other functions, including oxidative phosphorylation, transport across membrane, and mRNA transport and processing. Interestingly, we also uncovered widespread alterations in the maternal (i.e. non transcribed) mRNA population of SCNT embryos. We conclude that gene expression in early SCNT embryos is grossly abnormal, and that this is at least in part the result of incomplete reprogramming of transcription factor genes.
Keywords: embryo kind comparison
 
Overall design for both 1 and 2 cell stage we analyzed 4 samples each of fertilized embryos cultured with and without a-amanitin, scnt embryos cultured with and without a-amanitin, and parthennogenic embryos.
 
Contributor(s) Vassena R, Han Z, Gao S, Baldwin DA, Schultz RM, Latham KE
Citation(s) 17234177
Submission date Dec 21, 2006
Last update date Feb 11, 2019
Contact name Keith E Latham
URL http://www.temple.edu/lathamlab
Organization name Temple University School of Medicine
Department The Fels Institute for Cancer Research and Molecular Biology
Street address 3307 N Broad St
City Philadelphia
State/province PA
ZIP/Postal code 19140
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (40)
GSM149821 B6D2 1CELL FERTILIZED EMBRYO SAMPLE4
GSM149822 B6D2 1CELL SCNT EMBRYO SAMPLE A2
GSM149823 B6D2 1CELL SCNT EMBRYOS SAMPLE A1
Relations
BioProject PRJNA98891

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6595_RAW.tar 146.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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