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Series GSE65695 Query DataSets for GSE65695
Status Public on May 01, 2015
Title Genome-wide binding profiles of nuclear FGFR1, RXRα and Nur77 in pluripotent mouse Embryonic Stem Cells and during Retinoic Acid-induced differentiation
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We report the genome-wide binding patterns of nuclear FGFR1, RXRα and Nur77 in pluripotenet mESC and as mESC differentiate towared a neuronal lineage in response to high levels of Retinoic Acid treatment in vitro. We also report the genome-wide incorporation of histone varant H3.3 into chromatin specifically during Retinoic Acid-induced differentiation. This study presents nuclear FGFR1 as a global factor that controls genomic function by binding within the promoters of thousands of genes, both alone and in cooperation with RXR, Nur77 and histone H3.3, by targeting consensus DNA sequences of diverse transcription factor families. As such, it identifies a previously unknown, multifaceted form of control of major ontogenic pathways and gene networks targeted by the nuclear form of FGFR1.
 
Overall design Examination of nuclear FGFR1, RXRα, and Nur77 binding sites in 2 celluar states. Examination of H3.3 incorporation in 1 cellular state.
 
Contributor(s) Stachowiak M
Citation(s) 25923916, 33396256
Submission date Feb 06, 2015
Last update date Jan 19, 2021
Contact name Michal Stachowiak
E-mail(s) mks4@buffalo.edu
Phone 716-829-3540
Organization name State University of New York at Buffalo
Department Pathology and Anathomical Sciences
Street address 3435 Main Street
City Buffalo
State/province NY
ZIP/Postal code 14214
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (8)
GSM1603268 FGFR1(LIF)_ChIP_Seq
GSM1603269 RXRα(LIF)_ChIP_Seq
GSM1603270 Nur77(LIF)_ChIP_Seq
This SubSeries is part of SuperSeries:
GSE65698 Pluripotent mouse Embryonic Stem Cells and during Retinoic Acid-induced differentiation
Relations
BioProject PRJNA274739
SRA SRP053288

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65695_RAW.tar 3.9 Mb (http)(custom) TAR (of NARROWPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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