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Series GSE65573 Query DataSets for GSE65573
Status Public on Feb 04, 2015
Title Immortalized Human Hepatocyte (IHH) transcriptome response to wild-type Ebola viruses Zaire Ebola (ZEBOV '76) and Reston Ebola (REBOV '08)
Organism Homo sapiens
Experiment type Expression profiling by array
Non-coding RNA profiling by array
Summary The purpose of this experiment was to obtain samples for transcriptome analysis using wild-type viruses: Zaire Ebola (ZEBOV '76) and Reston Ebola (REBOV '08).
 
Overall design Overview of Experiment: Cells: Immortalized Human Hepatocytes (IHH); seed 60,000 cells per well in a 24-well plate. Infected with a multiplicity of infection (MOI) of 0.5. After infection, 3x wash with PBS and replace with 5% FCS DMEM without NaPyr or NEAA. Time matched mocks done in triplicate from same cell stock as rest of samples. Time Points = 0, 8, 24, 48, and 72 hrs post infection in triplicate. (For miRNA: 8, 24, 48, 72 hours.)
 
Contributor(s) Kawaoka Y, Waters KM, Halfmann P, Eisfeld AJ
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Feb 03, 2015
Last update date Jan 09, 2018
Contact name Natalie Heller
E-mail natalie.heller@pnnl.gov
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
 
Platforms (2)
GPL13497 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version)
GPL19730 Agilent-046064 Unrestricted_Human_miRNA_V19.0_Microarray (Probe Name version)
Samples (67)
GSM1600252 EIHH001_mock_8hr_1_microRNA
GSM1600253 EIHH001_mock_8hr_2_microRNA
GSM1600254 EIHH001_mock_8hr_3_microRNA
This SubSeries is part of SuperSeries:
GSE65575 Modeling Host Responses to Understand Severe Human Virus Infections
Relations
BioProject PRJNA274405

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65573_RAW.tar 180.0 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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