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Series GSE65529 Query DataSets for GSE65529
Status Public on Sep 07, 2015
Title Single cell analysis of macrophages exposed to beads coated with LPS from Salmonella enterica subsp. typhimurium
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We present a detailed single cell analysis of the macrophage response to LPS from Salmonella enterica. By combining single cell transcriptional analysis, fluorescently labeled, LPS-coated beads, and cytometry we are able to distinguish the responses of macrophages that have internalized LPS-coated beads and those that have not.
Overall design Analysis of 96 single macrophages that were either: left untreated, were exposed to but did not internalize uncoated beads, were exposed to and internalized uncoated beads, were exposed to but did not internalize LPS-coated beads, or were exposed to and did internalize LPS-coated beads.
Contributor(s) Avraham R, Haseley N, Brown D, Penaranda C, Jijon HB, Trombetta JJ, Satija R, Shalek AK, Xavier R, Regev A, Hung DT
Citation(s) 26343579
Submission date Feb 02, 2015
Last update date Mar 21, 2019
Contact name Deborah T Hung
Organization name Broad Institute
Department Infectious Disease Initiative
Lab Hung lab
Street address 415 Main Street
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (96)
GSM1599706 macrophage (exposed to S. enterica LPS) 1
GSM1599707 macrophage (exposed to S. enterica LPS) 2
GSM1599708 macrophage (exposed to S. enterica LPS) 3
BioProject PRJNA274281
SRA SRP053054

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65529_LPS_Counts_table.txt.gz 1.4 Mb (ftp)(http) TXT
GSE65529_LPS_TPM_table.txt.gz 1.9 Mb (ftp)(http) TXT
Raw data are available in SRA
Processed data is available on Series record

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