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| Status |
Public on Nov 30, 2015 |
| Title |
Human Embryo Single Cell Transcriptome Analysis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We sorted 2PN stage human embryos by predicted viability using a noninvasive measurement we previously developed. We then performed RNA-seq on each embryo (which was a single cell) and looked for differences in expression between viable and nonviable embryos.
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| |
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| Overall design |
We sequenced the transcriptomes of 22 human zygotes (2PN stage) embryos, of which 11 were predicted nonviable and 11 were predicted viable. We excluded 5 which had no siblings in this data set. From the remaining 17 embryos, we analyzed the set of genes that was differentially expressed between those predicted viable and those predicted nonviable.
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| |
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| Contributor(s) |
Yanez LZ, Han J, Behr BB, Reijo Pera RA, Camarillo DB |
| Citation(s) |
26904963 |
| |
| Submission date |
Jan 30, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Livia Zarnescu Yanez |
| E-mail(s) |
livia.z.yanez@gmail.com
|
| Organization name |
Stanford University
|
| Department |
Bioengineering
|
| Lab |
Camarillo Lab
|
| Street address |
443 Via Ortega
|
| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA274140 |
| SRA |
SRP053004 |
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