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Series GSE65393 Query DataSets for GSE65393
Status Public on Feb 01, 2015
Title Pseudomonas aeruginosa PA14 differential gene expression in bqsR (PA14_29730) mutants
Organism Pseudomonas aeruginosa
Experiment type Expression profiling by high throughput sequencing
Summary Before and after anaerobic Fe(II) shocked WT and ∆bqsR of late stationary phase P. aeruginosa PA14 strains
Associated publication: Kreamer NN, Costa F, Newman DK. 2015. The ferrous iron-responsive BqsRS two-component system activates genes that promote cationic stress tolerance. mBio 6(1):e02549-14. doi:10.1128/mBio.02549-14.
 
Overall design Expression profiles of rRNA-depleted total RNA from WT and ∆bqsR Fe(II)-shocked (before and after 30 min incubation with 200 µM ferrous ammonium sulfate ) cultures grown anaerobically to deep stationary phase (A500 = 0.8) in Fe-limited (1 µM ferrous ammonium sulfate) MOPS minimal medium containing succinate as the carbon source, in triplicate
 
Contributor(s) Kreamer NN, Newman DK
Citation(s) 25714721
Submission date Jan 28, 2015
Last update date May 15, 2019
Contact name Dianne K Newman
E-mail(s) dkn@caltech.edu
Organization name Caltech
Department BBE and GPS
Lab Dianne Newman
Street address 1200 E California Blvd
City Pasadena
State/province CA
ZIP/Postal code 91125
Country USA
 
Platforms (1)
GPL18287 Illumina HiSeq 2500 (Pseudomonas aeruginosa)
Samples (12)
GSM1595229 wild type, before shock, replicate 1
GSM1595230 wild type, before shock, replicate 2
GSM1595231 wild type, before shock, replicate 3
Relations
BioProject PRJNA273872
SRA SRP052915

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65393_150126_FDR_1_all_conditions_normalized_wt_0_gene_analysis.txt.gz 296.4 Kb (ftp)(http) TXT
GSE65393_150126_FDR_1_all_conditions_normalized_wt_0_txn_unit_analysis.txt.gz 195.0 Kb (ftp)(http) TXT
GSE65393_BqsR_regulon_genes.txt.gz 1.7 Kb (ftp)(http) TXT
GSE65393_BqsR_regulon_txn_unit.txt.gz 1.6 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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