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Series GSE6521 Query DataSets for GSE6521
Status Public on Dec 15, 2006
Title MCF7 inhibitor
Organism Homo sapiens
Experiment type Expression profiling by array
Summary ErbB receptor ligands, epidermal growth factor (EGF) and heregulin (HRG), induce dose-dependent transient and sustained intracellular signaling, proliferation and differentiation of MCF-7 breast cancer cells, respectively. In an effort to delineate the ligand-specific cell determination mechanism, we investigated time-course gene expressions induced by EGF and HRG that induce distinct cellular phenotypes in MCF-7 cells. To analyze the effects of ligand dosage and time for the gene expression independently, we developed a statistical method for decomposing the expression profiles into the two effects. Our results indicated that signal transduction pathways devotedly convey quantitative properties of the dose-dependent activation of ErbB receptor to early transcription. The results also implied that moderate changes in the expression levels of numbers of genes, not the predominant regulation of a few specific genes, might cooperatively work at the early stage of the transcription for determining the cell fate. However, the EGF- and HRG-induced distinct signal durations resulted in the ligand-oriented biphasic induction of proteins after 20 min. The selected gene list and HRG-induced prolonged signaling suggested that transcriptional feedback to the intracellular signaling results in a graded to biphasic response in the cell determination process, and that each ErbB receptor is inextricably responsible for the control of amplitude and duration of cellular biochemical reactions.
Keywords: time course
Overall design MCF-7 human breast cancer cells were incubated from 5min to 90min after administration of the growth hormone (heregulin (HRG)), the kinase inhibitor (U0126 (a MEK inhibitor), AG1478 (an EGFR kinase inhibitor) or both growth hormone and one of kinase inhibitor. Control was set as growth hormone/inhibitor non-treated cells. For each inhibitor, one control was used.
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Submission date Dec 13, 2006
Last update date Jan 08, 2019
Contact name Mariko Okada
Organization name RIKEN RCAI
Lab Laboratory for Cellular Systems Modeling
Street address W518, 1-7-22, Suehiro-cho, Tsurumi-ku
City Yokohama
ZIP/Postal code 230-0045
Country Japan
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (37)
GSM149913 MCF7_inhibitor_control_for_U0126
GSM149914 MCF7_inhibitor_control_for_AG1478
GSM149915 MCF7_inhibitor_HRG_5min
BioProject PRJNA98795

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE6521_RAW.tar 163.6 Mb (http)(custom) TAR (of CEL, EXP)
Raw data provided as supplementary file

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