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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 30, 2016 |
| Title |
ChIP-seq analysis of Arid1a and C/ebpα in the mouse livers |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Arid1a is the subunit of SWI/SNF complex, which was reported to guide SWI/SNF to DNA. Here, we found that loss of Arid1a in the liver and other adult tissues results in improved organ regeneration. Within SWI/SNF complexes, Arid1a physically interacts with C/ebpα, a hepatocyte transcription factor that drives maturation and limits proliferation. Genome-wide analysis showed that loss of Arid1a reduces the recruitment and activity of C/ebpα on target promoters, resulting in expression programs that favor regeneration and cellular fitness during injury. Arid1a binding is enriched in promoters near transcriptional start sites (TSSs), and C/ebpα binds at precisely the same positions, indicating that Arid1a facilitates C/ebpα binding across the genome.
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| Overall design |
Perfuse and isolate primary hepatocytes from mice livers, analysis of genomic occupancy of C/ebpα and H3K4me2 in hepatocytes from Arid1a WT and Arid1a liver specific KO mice by ChIP-seq. Analysis of genomic occupancy of Arid1a in the hepatocytes from V5-Arid1a transgenic mouse by ChIP-seq.
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| Contributor(s) |
Sun X, Xing C, Zhu H |
| Citation(s) |
27044474 |
| Submission date |
Jan 21, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Xuxu Sun |
| Organization name |
shanghai jiao tong university
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| Department |
school of medicine
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| Street address |
227 south chongqing road
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| City |
shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
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| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA273315 |
| SRA |
SRP052719 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE65167_RAW.tar |
1.2 Gb |
(http)(custom) |
TAR (of BED, BIGWIG, BROADPEAK) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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