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Series GSE64842 Query DataSets for GSE64842
Status Public on Jan 10, 2015
Title Rat Life Cycle Kidney miRNA Expression Data
Organism Rattus norvegicus
Experiment type Non-coding RNA profiling by array
Summary Kidney miRNA expression was examined in F344 rats at 2, 5, 6, 8, 15, 21, 78, and 104 weeks of age in both sexes using Agilent miRNA microarrays. 311 miRNAs were found to be expressed in at least one age and sex. Filtering criteria of ≥1.5 fold change and ANOVA (FDR <5%) revealed 174 differentially expressed miRNAs in the kidney; 173 and 34 miRNAs exhibiting age and sex effects, respectively. Principal component analysis revealed age effects predominated over sex effects, with 2 week miRNA expression being much different from other ages. No significant sexually dimorphic miRNA expression was observed from 5 to 8 weeks, while the most differential expression (13 miRNAs) was observed at 21 weeks. Potential target genes of these differentially expressed miRNAs were identified. Pathway analysis was used to investigate the possible roles of these target genes in age- and sex-specific differences.
 
Overall design Untreated male and female F344 rats from 2, 5, 6, 8, 15, 21, 78, and 104 weeks of age (n=5) were sacrificed by CO2 asphyxiation, whole kidneys collected and homogenized, total RNA, including small RNA fraction, was used for miRNA expression arrays (Agilent).
 
Contributor(s) Kwekel JC
Citation(s) 25653823
Submission date Jan 09, 2015
Last update date May 22, 2017
Contact name Vikrant Vijay
E-mail(s) vikrant.vijay@fda.hhs.gov
Phone 870-543-7525
Organization name US FDA National Center for Toxicological Research
Department Division of Systems Biology
Lab Personalized Medicine
Street address 3900 NCTR Road
City Jefferson
State/province AR
ZIP/Postal code 72079
Country USA
 
Platforms (1)
GPL14860 Agilent-031189 Unrestricted_Rat_miRNA_v16.0_Microarray (miRNA ID version)
Samples (74)
GSM1581430 kidney_104WK_female_rep1
GSM1581431 kidney_104WK_female_rep2
GSM1581432 kidney_104WK_female_rep3
Relations
BioProject PRJNA272146

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64842_RAW.tar 56.3 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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