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Series GSE64673 Query DataSets for GSE64673
Status Public on Jan 07, 2015
Title Low-cost, low-input RNA-seq protocols perform nearly as well as high-input protocols
Organisms Drosophila melanogaster; Drosophila virilis
Experiment type Expression profiling by high throughput sequencing
Summary We sequenced mRNA according to several library prep protocols with known mixtures of two species of Drosophila in order to establish linear response in each protocol.
 
Overall design For each library prep protocol, mixtures with 0%, 5%, 10%, and 20% D. virilis total RNA was prepared, then libraries prepared according to instructions.
 
Contributor(s) Combs PA, Eisen MB
Citation(s) 25834775
Submission date Jan 05, 2015
Last update date Aug 01, 2018
Contact name Peter Acuña Combs
E-mail pcombs@stanford.edu
Organization name Stanford University
Department Biology
Lab Hunter Fraser
Street address Gilbert Rm 309, 371 Serra Mall
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (1)
GPL19602 Illumina HiSeq 2000 (Drosophila melanogaster; Drosophila virilis)
Samples (26)
GSM1576711 Clontech - 0%
GSM1576712 Clontech - 5%
GSM1576713 Clontech - 10%
Relations
BioProject PRJNA271674
SRA SRP051726

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64673_summary.tsv.gz 644.9 Kb (ftp)(http) TSV
GSE64673_summary_in_subset_min.tsv.gz 501.6 Kb (ftp)(http) TSV
Raw data are available in SRA
Processed data is available on Series record

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