|
| Status |
Public on Jan 07, 2015 |
| Title |
Low-cost, low-input RNA-seq protocols perform nearly as well as high-input protocols |
| Organisms |
Drosophila melanogaster; Drosophila virilis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We sequenced mRNA according to several library prep protocols with known mixtures of two species of Drosophila in order to establish linear response in each protocol.
|
| |
|
| Overall design |
For each library prep protocol, mixtures with 0%, 5%, 10%, and 20% D. virilis total RNA was prepared, then libraries prepared according to instructions.
|
| |
|
| Contributor(s) |
Combs PA, Eisen MB |
| Citation(s) |
25834775 |
| |
| Submission date |
Jan 05, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Peter Acuña Combs |
| E-mail(s) |
pcombs@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Biology
|
| Lab |
Hunter Fraser
|
| Street address |
Gilbert Rm 309, 371 Serra Mall
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19602 |
Illumina HiSeq 2000 (Drosophila melanogaster; Drosophila virilis) |
|
| Samples (26)
|
|
| Relations |
| BioProject |
PRJNA271674 |
| SRA |
SRP051726 |
Less...
More...