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Series GSE64655 Query DataSets for GSE64655
Status Public on Jan 06, 2015
Title A Cell-based Systems Biology Assessment of Human Blood to Monitor Immune Responses After Influenza Vaccination
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Systems biology is an approach to comprehensively study complex interactions within a biological system. Most published systems vaccinology studies have utilized whole blood or peripheral blood mononuclear cells (PBMC) to monitor the immune response after vaccination. Because human blood is comprised of multiple hematopoietic cell types, the potential for masking responses of under-represented cell populations is increased when analyzing whole blood or PBMC. To investigate the contribution of individual cell types to the immune response after vaccination, we established a rapid and efficient method to purify human T and B cells, natural killer (NK) cells, myeloid dendritic cells (mDC), monocytes, and neutrophils from fresh venous blood. Purified cells were fractionated and processed in a single day. RNA-Seq and quantitative shotgun proteomics were performed to determine expression profiles for each cell type prior to and after inactivated seasonal influenza vaccination. Our results show that transcriptomic and proteomic profiles generated from purified immune cells differ significantly from PBMC. Differential expression analysis for each immune cell type also shows unique transcriptomic and proteomic expression profiles as well as changing biological networks at early time points after vaccination. This cell type-specific information provides a more comprehensive approach to monitor vaccine responses.
Overall design PBMC and six purified cell types from two vaccinated donors were isolated prior to (d0) and at days 1, 3, and 7 post-TIV vaccination for RNA-seq analysis
Contributor(s) Hoek KL, Link AJ
Citation(s) 25706537
Submission date Jan 05, 2015
Last update date May 15, 2019
Contact name Andrew J Link
Phone 615-343-6823
Organization name Vanderbilt University School of Medicine
Department Pathology, Microbiology and Immunology
Lab Andrew Link Lab
Street address 1161 21st Ave South
City Nashville
State/province Tennessee
ZIP/Postal code 37232-2363
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (56)
GSM1576391 HD30_B_0_SL27200
GSM1576392 HD30_B_1_SL27207
GSM1576393 HD30_B_3_SL27214
BioProject PRJNA271578
SRA SRP051688

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Supplementary file Size Download File type/resource
GSE64655_Normalized_transcript_expression_in_human_immune_cells.txt.gz 6.9 Mb (ftp)(http) TXT
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