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Series GSE64586 Query DataSets for GSE64586
Status Public on Dec 01, 2015
Organism Homo sapiens
Experiment type Expression profiling by array
Summary High expression of the FOXP1 transcription factor distinguishes the highly aggressive Activated B Cell (ABC) type of Diffuse Large B Cell Lymphoma (DLBCL) from the more indolent Germinal Center (GCB) DLBCL subtype and is correlated with poor prognosis. A genetic or functional role for FOXP1 in lymphomagenesis and/or tumor maintenance, however, remains unknown. Here, we report that sustained expression of FOXP1 is necessary for ABC DLBCL cell line survival. Genome-wide transcript profiling reveals that FOXP1 acts directly and indirectly by enforcing expression of known ABC DLBCL hallmarks, including the classical NF-kappaB survival pathway. Our data further suggest that FOXP1 maintains the ABC subtype distinction by repressing gene expression programs dominant in GCB DLBCL and supports a model in which the target of ABC DLBCL transformation is a transitory cell type en route from the germinal center B cell to the terminally differentiated plasma cell.
Overall design HBL1 (n=12) or TMD8 (n=12) cells were infected with either a Ctrl shRNA or with shRNAs targeting FOXP1. The shRNA expression was induced for 1, 2, 3, or 4 days.
Citation(s) 26787899
Submission date Dec 30, 2014
Last update date Feb 22, 2018
Contact name Louis M. Staudt
Phone 301-402-1892
Organization name National Cancer Institute
Department Lymphoid Malignancies Branch
Lab Louis M Staudt
Street address 9000 Rockville Pike, Bldg 10, Rm 4N114
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (24)
GSM1574716 TMD8 shFOXP1 26 - day 1 - mAdbID:104007
GSM1574717 TMD8 shFOXP1 26 - day 2 - mAdbID:104008
GSM1574718 TMD8 shFOXP1 26 - day 3 - mAdbID:104009
BioProject PRJNA271357

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64586_RAW.tar 356.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table

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