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Series GSE64453 Query DataSets for GSE64453
Status Public on Sep 08, 2015
Title Transformation of enriched mammary cell populations with Polyoma Middle T Antigen influences tumor subtype and metastatic potential
Organism Mus musculus
Experiment type Expression profiling by array
Summary Breast cancer is a multifaceted disease, exhibiting significant molecular, histological, and pathological diversity. Factors that impact this heterogeneity are poorly understood; however, transformation of distinct normal cell populations of the breast may generate different tumor phenotypes. Our previous study demonstrates that the polyomavirus middle T antigen (PyMT) oncogene can establish diverse tumor subtypes when broadly expressed within mouse mammary epithelial cells. Herein, we assess the molecular, histological, and metastatic outcomes from distinct mammary cell populations transformed with PyMT. By combining several methodologies, including lentiviral infection, cell sorting, and transplantation, we have characterized tumors arising from enriched populations of mammary epithelial cells. We have found that expression of PyMT within different cell populations influences tumor histology, molecular subtype, and metastatic potential.
 
Overall design 32 samples, 1 from each of 32 mouse mammary tumors arising from transplanted mouse mamary epithelial cells (MMECs) transduced with PyMT-expressing lentivirus. MMECs were sorted into four different types prior to transplant: luminal CD133+ (8 samples), luminal CD133- (11 samples), stem (6 samples), and basal (7 samples). The background for the cell donor and transplant recipients mice was FVB/NJ obtained from Jackson Laboratories.
 
Contributor(s) Drobysheva D, Smith BA, Ekiz HA, Welm BE
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Submission date Dec 22, 2014
Last update date Jan 12, 2017
Contact name Brett Milash
E-mail brett.milash@utah.edu
Organization name University of Utah
Department Center for High Performance Computing
Street address 155 S. 1452 E. Room 414
City Salt Lake City
State/province UT
ZIP/Postal code 84112
Country USA
 
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (32)
GSM1571348 luminal_CD133_positive_2sort7
GSM1571349 stem_2sort8
GSM1571350 luminal_CD133_positive_2sort21
Relations
BioProject PRJNA271037

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64453_RAW.tar 295.3 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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