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Series GSE64357 Query DataSets for GSE64357
Status Public on Sep 08, 2015
Title Knockout mice reveal an essential role for Epithelial splicing regulatory proteins (Esrps) in mammalian development and epithelial splicing in vivo
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Tissue- and cell-type specific regulators of alternative splicing (AS) are an essential layer of posttranscriptional gene regulation necessary for normal cellular function, patterning, and development. Here we report the Epithelial splicing regulatory proteins (Esrps) are required for patterning of multiple organs, with loss of both paralogs, Esrp1 and Esrp2, resulting in increasingly severe phenotypes. Global profiling of the Esrp splicing regulatory network from total epidermis revealed varied splicing sensitivity of Esrp targets upon loss of Esrp1 or double knockout. This may explain the progressive phenotypes seen in Esrp knockout mice, and these mice provide a unique genetic tool to evaluate functional consequences of epithelial splicing events in vivo.
Overall design RNA from purified total epidermis (basal keratinocyte layer to cornified layer) of E18.5 mouse embryos were harvested by Trizol extraction. (Esrp1+/+, Esrp2-/- (n=2), Esrp1-/-, Esrp2+/+ (n=3), Esrp1-/-, Esrp2+/- (n=2), and Esrp1-/-, Esrp2-/- (n=2). 1 ug of total RNA was used for for RNA-seq library preparation using the TruSeqâ„¢ Stranded mRNA LT Sample Prep Kit (Illumina). 100x2 bp paired-end RNA-seq reads were generated on a HiSeq 2000 sequencer.
Contributor(s) Xing Y, Carstens R
Citation(s) 26371508
Submission date Dec 19, 2014
Last update date May 15, 2019
Contact name Yi Xing
Organization name UCLA
Department Department of Microbiology, Immunology, & Molecular Genetics
Street address 650 Charles E. Young Dr. South
City Los Angeles
State/province CA
ZIP/Postal code 90095-7278
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (9)
GSM1569076 DKO, rep1
GSM1569077 DKO, rep2
GSM1569078 KH, rep1
BioProject PRJNA270823
SRA SRP051370

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Supplementary file Size Download File type/resource
GSE64357_FPKMs.Epidermis.Mouse.txt.gz 1.7 Mb (ftp)(http) TXT
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