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| Status |
Public on Feb 18, 2015 |
| Title |
High-throughput RNA sequencing of methanosarcina grown on methylated sulfur compounds |
| Organism |
Methanosarcina acetivorans C2A |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Methylated sulfur compounds, including dimethylsulfide (DMS), methylmercaptopropionic acid (MMPA) and methylsulfide (MeSH), are well-documented to play roles in global sulfur cycle and climate homeostasis, yet the molecular mechanisms of how they are metabolized by methanogens remain largely uncharacterized. Here, using high-throughtput sequencing of RNA (RNA-seq), we gained insight into how methanogens respond to methylated sulfur compounds at the transcriptional level.
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| Overall design |
The mRNA from wild-type of Methanosarcina acetivorans C2A grown on methylated sulfur compounds were harvested, sequenced and mapped to the genome. Then, we compared RNA-seq profiles to that grown on MeOH in search of unque genes.
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| |
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| Contributor(s) |
Fu H, Kohler PR, Metcalf WW |
| Citation(s) |
25691524 |
| |
| Submission date |
Dec 19, 2014 |
| Last update date |
Oct 28, 2022 |
| Contact name |
He Fu |
| E-mail(s) |
biofuhe@gmail.com
|
| Organization name |
University of Georgia
|
| Department |
Microbiology
|
| Street address |
120 Cedar St
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30602 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19569 |
Illumina HiSeq 2000 (Methanosarcina acetivorans C2A) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA270832 |
| SRA |
SRP051376 |
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