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Series GSE64247 Query DataSets for GSE64247
Status Public on Apr 29, 2015
Title The kinase DYRK1A reciprocally regulates the differentiation of Th17 and regulatory T cells [array]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Using an unbiased chemical biology approach, we discover harmine as a novel regulator of Treg/Th17 differentiation. Harmine enhances Treg differentiation (working in conjunction with low levels of exogenous TGFb) and inhibits Th17 differentiation. Analysis of global gene expression of Tregs generated using low TGFb + harmine reveals significant similarity to Tregs generated using high TGFb only and suggests relevance of harmine-engaged mechanisms to IBD.
Overall design Naïve CD4+CD62L+ T cells were purified from Foxp3GFP mice either by MACS or FACS, then cultured in either low TGFb + harmine or high TGFb conditions. GFP+ Tregs were sorted by FACS at day 4.
Contributor(s) Khor B, Goel G, Xavier RJ
Citation(s) 25998054
Submission date Dec 16, 2014
Last update date Jan 16, 2019
Contact name Bernard Khor
Organization name Massachusetts General Hospital
Department Pathology
Lab Xavier
Street address 185 Cambridge St MCPZN 7106
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (12)
GSM1567016 Naive_FACS_1
GSM1567017 Naive_FACS_2
GSM1567018 Naive_MACS_1
This SubSeries is part of SuperSeries:
GSE67961 The kinase DYRK1A reciprocally regulates the differentiation of Th17 and regulatory T cells
BioProject PRJNA270532

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64247_RAW.tar 15.8 Mb (http)(custom) TAR
GSE64247_non_normalized.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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