|
| Status |
Public on Jun 19, 2015 |
| Title |
Nucleoporin Nup153 Regulates Stem Cell Pluripotency through Gene Silencing |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Nucleoporins (Nups) are a family of proteins best known as the constituent building blocks of nuclear pore complexes (NPCs), the transport channels that mediate nuclear transport. Recent evidence suggest that several Nups have additional roles in controlling the activation and silencing of developmental genes, however, the mechanistic details of these functions remain poorly understood. Here, we show that depletion of Nup153 in mouse embryonic stem cells (mESCs) causes the de-repression of developmental genes and induction of early differentiation. This loss of pluripotency is not associated with defects in global nucleo-cytoplasmic transport activity. Instead, Nup153 binds to the transcriptional start site (TSS) of developmental genes and mediates the recruitment of the polycomb repressive complex 1 (PRC1) to its target loci. Our results reveal a nuclear transport-independent role of Nup153 in maintaining stem cell pluripotency and introduce a role of NPC proteins in mammalian epigenetic gene silencing.
|
| |
|
| Overall design |
RNA-seq, ChIP-Seq, and DamID-Seq for Nup153, Oct4, and key chromatin regulators in mouse ES cells and neural progenitors
|
| |
|
| Contributor(s) |
Jacinto FV, Benner C, Hetzer MW |
| Citation(s) |
26080816 |
| |
| Submission date |
Dec 09, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Christopher Benner |
| E-mail(s) |
cbenner@ucsd.edu
|
| Organization name |
University of California, San Diego (UCSD)
|
| Department |
Medicine
|
| Street address |
9500 Gilman Dr. MC 0640
|
| City |
La Jolla |
| State/province |
California |
| ZIP/Postal code |
92093-0640 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (19)
|
|
| Relations |
| BioProject |
PRJNA269747 |
| SRA |
SRP050985 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE64008_Cbx7.shCtrl.bed.gz |
164.9 Kb |
(ftp)(http) |
BED |
| GSE64008_Nup153.DamID.bed.gz |
47.9 Kb |
(ftp)(http) |
BED |
| GSE64008_Oct4.DamID.bed.gz |
48.9 Kb |
(ftp)(http) |
BED |
| GSE64008_Oct4.ctrl.bed.gz |
19.2 Kb |
(ftp)(http) |
BED |
| GSE64008_Oct4.shNup153.bed.gz |
20.9 Kb |
(ftp)(http) |
BED |
| GSE64008_Ring1B.NeuP.bed.gz |
193.2 Kb |
(ftp)(http) |
BED |
| GSE64008_Ring1B.shCtrl.bed.gz |
151.2 Kb |
(ftp)(http) |
BED |
| GSE64008_Ring1B.shNup153-2.bed.gz |
208.0 Kb |
(ftp)(http) |
BED |
| GSE64008_Ring1B.shNup153-4.bed.gz |
155.1 Kb |
(ftp)(http) |
BED |
| GSE64008_fpkm.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
Less...
More...