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Series GSE63917 Query DataSets for GSE63917
Status Public on May 20, 2015
Title The carbonic anhydrase inhibitor ethoxzolamide inhibits the Mycobacterium tuberculosis PhoPR regulon and Esx-1 secretion and attenuates virulence
Organism Mycobacterium tuberculosis
Experiment type Expression profiling by high throughput sequencing
Summary The purpose of this study was to examine how ethoxzolamide modulates gene M. tuberculosis gene expression at acidic pH. We observed that ethoxzolamide downregulates genes of the PhoPR regulon.
Overall design Mtb strain CDC1551 was grown at 37C in T-25 vented, standing tissue culture flasks in 8 ml of buffered 7H9 medium seeded an initial OD of 0.2. Three conditions were examined with two biological replicates: 1) DMSO treated, pH 7.0, 2) DMSO treated, pH 5.7, 3) 40 ┬ÁM ethoxzolamide treated, pH 5.7. The CDC1551(phoP::Tn) mutant was grown in a similar manner and treated with DMSO at pH 7.0 and 5.7. Following six days incubation, total bacterial RNA was extracted and analyzed as previously described.
Contributor(s) Johnson BK, Colvin CJ, Abramovitch RB
Citation(s) 25987613
Submission date Dec 05, 2014
Last update date Sep 06, 2018
Contact name Robert B. Abramovitch
Organization name Michigan State University
Department Microbiology and Molecular Genetics
Street address 567 Wilson Road
City East Lansing
State/province MI
ZIP/Postal code 48824
Country USA
Platforms (1)
GPL17879 Illumina HiSeq 2500 (Mycobacterium tuberculosis)
Samples (8)
GSM1560341 WT, DMSO, pH 7.0, Rep A
GSM1560342 WT, DMSO, pH 7.0, Rep B
GSM1560343 WT, DMSO, pH 5.7, Rep A
BioProject PRJNA269492
SRA SRP050863

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE63917_SupplementalTable1A_DMSO_pH5.7_v_pH7_induction.txt.gz 8.2 Kb (ftp)(http) TXT
GSE63917_SupplementalTable1B_DMSO_pH5.7_v_pH7_repression.txt.gz 11.0 Kb (ftp)(http) TXT
GSE63917_SupplementalTable2A_ETZ_v_DMSO_pH5.7_induction.txt.gz 782 b (ftp)(http) TXT
GSE63917_SupplementalTable2B_ETZ_v_DMSO_pH5.7_repression.txt.gz 3.2 Kb (ftp)(http) TXT
GSE63917_SupplementalTable3A_PhoPTn_v_DMSO_pH5.7_induction.txt.gz 4.8 Kb (ftp)(http) TXT
GSE63917_SupplementalTable3B_PhoPTn_v_DMSO_pH5.7_repression.txt.gz 11.8 Kb (ftp)(http) TXT
Raw data are available in SRA
Processed data is available on Series record

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