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Series GSE63871 Query DataSets for GSE63871
Status Public on Sep 15, 2015
Title Smad2 and Smad3 binding sites in H345-TbRII cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Transforming growth factor (TGF)-beta induces apoptosis of many types of cancer cells and acts as a tumor suppressor. We found lower expression of TGF-beta type II receptor (TbRII) in most of SCLC cells and tissues than in normal lung epithelial cells and normal lung tissues, respectively. In vitro cell growth and in vivo tumor formation were suppressed by TGF-beta-mediated apoptosis when the wild-type TbRII was overexpressed in SCLC cells. We therefore determined Smad2 and Smad3 (Smad2/3) binding sites in a SCLC cell line H345 stably expressing exogenous TbRII (H345-TbRII) to identify target genes of TGF-beta.
Overall design Smad2 and Smad3 binding sites in H345-TbRII cells were determined by ChIP-seq (one sample analysis, without replicates).
Contributor(s) Murai F, Ehata S, Koinuma D, Miyazono K
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Submission date Dec 04, 2014
Last update date May 15, 2019
Contact name Daizo Koinuma
Organization name University of Tokyo
Department Molecular Pathology
Street address Hongo 7-3-1, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-0033
Country Japan
Platforms (1)
GPL17301 Ion Torrent PGM (Homo sapiens)
Samples (1)
GSM1558744 Smad2 and Smad3 ChIP-seq of H345-TbRII cells
BioProject PRJNA269296
SRA SRP050562

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Supplementary file Size Download File type/resource
GSE63871_RAW.tar 131.6 Mb (http)(custom) TAR (of BED, WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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