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Series GSE63854 Query DataSets for GSE63854
Status Public on Dec 05, 2014
Title Global Genome Analysis of the Downstream Binding Targets of Testis Determining Factor SRY and SOX9 [SRY ChIP]
Organism Rattus norvegicus
Experiment type Genome binding/occupancy profiling by array
Summary A major event in mammalian male sex determination is the induction of the testis determining factor Sry and its downstream gene Sox9. The current study provides one of the first genome wide analyses of the downstream gene binding targets for SRY and SOX9 to help elucidate the molecular control of Sertoli cell differentiation and testis development. A modified ChIP-Chip analysis using a comparative hybridization was used to identify 71 direct downstream binding targets for SRY and 109 binding targets for SOX9. Interestingly, only 5 gene targets overlapped between SRY and SOX9. In addition to the direct response element binding gene targets, a large number of atypical binding gene targets were identified for both SRY and SOX9. Bioinformatic analysis of the downstream binding targets identified gene networks and cellular pathways potentially involved in the induction of Sertoli cell differentiation and testis development. The specific DNA sequence binding site motifs for both SRY and SOX9 were identified. Observations provide insights into the molecular control of male gonadal sex determination.
 
Overall design The current study provides one of the first genome wide analyses of the downstream gene binding targets for SRY and SOX9 to help elucidate the molecular control of Sertoli cell differentiation and testis development. At embryonic day 13 (E13) of pregnancy rats were euthanized and embryonic gonads were collected for chromatin. A modified ChIP-Chip analysis using a comparative hybridization was used to identify direct downstream binding targets for SRY and for SOX9. Then, bioinformatic analysis of the downstream binding targets was done to identify gene networks and cellular pathways that are potentially involved in the induction of Sertoli cell differentiation and testis development.
 
Contributor(s) Bhandari RK, Haque MM, Skinner MK
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Submission date Dec 04, 2014
Last update date Dec 05, 2014
Contact name Michael K Skinner
E-mail(s) skinner@mail.wsu.edu
Organization name WSU
Department SBS
Street address Abelson 507
City Pullman
State/province WA
ZIP/Postal code 99163
Country USA
 
Platforms (1)
GPL18610 NimbleGen Rat CpG Island Plus RefSeq Promoter 720k array [090618_RN34_CpG_Refseq_Prom_MeDIP]
Samples (3)
GSM1558507 Sry_2/ IgG_2a
GSM1558508 Sry_1/ IgG_1a
GSM1558509 Sry_3/ IgG_3a
This SubSeries is part of SuperSeries:
GSE63856 Global Genome Analysis of the Downstream Binding Targets of Testis Determining Factor SRY and SOX9
Relations
BioProject PRJNA269271

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE63854_RAW.tar 128.9 Mb (http)(custom) TAR (of PAIR)
GSE63854_RegionsCombined.csv.gz 181.5 Kb (ftp)(http) CSV
Raw data provided as supplementary file
Processed data are available on Series record

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