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Status |
Public on Dec 04, 2014 |
Title |
C.elegans treated with control or elt-2 RNAi during adulthood and exposed to E. coli or P. aeruginosa |
Organism |
Caenorhabditis elegans |
Experiment type |
Expression profiling by array
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Summary |
Transcriptional profiling of adult C.elegans exposed to E.coli or to GFP-expressing P. aeruginosa (strain PA14). For P. aeruginosa exposure, worms were separated into 2 groups - fully colonized (green) or non-colonized (dark).
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Overall design |
Six conditions ( control or elt-2 RNAi ; E.coli, P. aeurginosa colonized, and P. aeurginosa non-colonized). 2-color arrrays, each sample co-hybridized with the same reference RNA sample from mixed stage C.elegans cultures Worms were either sorted using the wormsorter (WS) or were hand picked (HP) under a fluorescent stereoscope
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Contributor(s) |
Twumasi-Boateng K, Shapira M |
Citation(s) |
26016853 |
Submission date |
Dec 04, 2014 |
Last update date |
Jan 08, 2020 |
Contact name |
Michael Shapira |
E-mail(s) |
mshapira@berkeley.edu
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Organization name |
UC Berkeley
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Department |
Integrative Biology
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Lab |
Dr. Michael Shapira Lab
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Street address |
3060 Valley Life Sciences Bldg room 5163
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City |
Berkeley |
State/province |
CA |
ZIP/Postal code |
94720-3140 |
Country |
USA |
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Platforms (1) |
GPL9458 |
Washington University/Genome Sequencing Center C. elegans 23K |
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Samples (14)
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GSM1558423 |
controlRNAi, E.coli, hand picked, rep 1 |
GSM1558424 |
controlRNAi, E.coli, worm sorter, rep 2 |
GSM1558425 |
controlRNAi, E.coli, worm sorter, rep 3 |
GSM1558426 |
controlRNAi, P. aeruginosa_non-colonized, hand picked, rep 1 |
GSM1558427 |
controlRNAi, P. aeruginosa_non-colonized, worm sorter, rep 2 |
GSM1558428 |
controlRNAi, P. aeruginosa_non-colonized, worm sorter, rep 3 |
GSM1558429 |
controlRNAi, P. aeruginosa_colonized, hand picked, rep 1 |
GSM1558430 |
controlRNAi, P. aeruginosa_colonized, worm sorter, rep 2 |
GSM1558431 |
controlRNAi, P. aeruginosa_colonized, worm sorter, rep 3 |
GSM1558432 |
elt-2RNAi, E.coli, hand picked, rep 1 |
GSM1558433 |
elt-2RNAi, E.coli, wormsorter, rep 2 |
GSM1558434 |
elt-2RNAi, P. aeruginosa_non-colonized, hand picked, rep 1 |
GSM1558435 |
elt-2RNAi, P. aeruginosa_non-colonized, worm sorter, rep 2 |
GSM1558436 |
elt-2RNAi, P. aeruginosa_colonized, hand picked |
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Relations |
BioProject |
PRJNA269251 |
Supplementary file |
Size |
Download |
File type/resource |
GSE63846_RAW.tar |
30.5 Mb |
(http)(custom) |
TAR (of GPR) |
Raw data provided as supplementary file |
Processed data included within Sample table |
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