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| Status |
Public on Dec 04, 2014 |
| Title |
C.elegans treated with control or elt-2 RNAi during adulthood and exposed to E. coli or P. aeruginosa |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
Transcriptional profiling of adult C.elegans exposed to E.coli or to GFP-expressing P. aeruginosa (strain PA14).
For P. aeruginosa exposure, worms were separated into 2 groups - fully colonized (green) or non-colonized (dark).
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| Overall design |
Six conditions ( control or elt-2 RNAi ; E.coli, P. aeurginosa colonized, and P. aeurginosa non-colonized).
2-color arrrays, each sample co-hybridized with the same reference RNA sample from mixed stage C.elegans cultures
Worms were either sorted using the wormsorter (WS) or were hand picked (HP) under a fluorescent stereoscope
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| Contributor(s) |
Twumasi-Boateng K, Shapira M |
| Citation(s) |
26016853 |
| Submission date |
Dec 04, 2014 |
| Last update date |
Jan 08, 2020 |
| Contact name |
Michael Shapira |
| E-mail(s) |
mshapira@berkeley.edu
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| Organization name |
UC Berkeley
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| Department |
Integrative Biology
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| Lab |
Dr. Michael Shapira Lab
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| Street address |
3060 Valley Life Sciences Bldg room 5163
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| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720-3140 |
| Country |
USA |
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| Platforms (1) |
| GPL9458 |
Washington University/Genome Sequencing Center C. elegans 23K |
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| Samples (14)
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| GSM1558423 |
controlRNAi, E.coli, hand picked, rep 1 |
| GSM1558424 |
controlRNAi, E.coli, worm sorter, rep 2 |
| GSM1558425 |
controlRNAi, E.coli, worm sorter, rep 3 |
| GSM1558426 |
controlRNAi, P. aeruginosa_non-colonized, hand picked, rep 1 |
| GSM1558427 |
controlRNAi, P. aeruginosa_non-colonized, worm sorter, rep 2 |
| GSM1558428 |
controlRNAi, P. aeruginosa_non-colonized, worm sorter, rep 3 |
| GSM1558429 |
controlRNAi, P. aeruginosa_colonized, hand picked, rep 1 |
| GSM1558430 |
controlRNAi, P. aeruginosa_colonized, worm sorter, rep 2 |
| GSM1558431 |
controlRNAi, P. aeruginosa_colonized, worm sorter, rep 3 |
| GSM1558432 |
elt-2RNAi, E.coli, hand picked, rep 1 |
| GSM1558433 |
elt-2RNAi, E.coli, wormsorter, rep 2 |
| GSM1558434 |
elt-2RNAi, P. aeruginosa_non-colonized, hand picked, rep 1 |
| GSM1558435 |
elt-2RNAi, P. aeruginosa_non-colonized, worm sorter, rep 2 |
| GSM1558436 |
elt-2RNAi, P. aeruginosa_colonized, hand picked |
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| Relations |
| BioProject |
PRJNA269251 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE63846_RAW.tar |
30.5 Mb |
(http)(custom) |
TAR (of GPR) |
| Raw data provided as supplementary file |
| Processed data included within Sample table |
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