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| Status |
Public on Aug 26, 2015 |
| Title |
RNA-seq analysis of flg22-induced gene expression changes in wild type, asr3-1 mutant and OX9 overexpression line |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The correlation coefficient (R) for the expression profile of all transcripts between WT and asr3-1, and between WT and OX9 without flg22 treatment is close to linear, suggesting that ASR3 does not affect general gene transcription. Hierarchical clustering analysis flg22-induced genes in any of the three genotypes suggested that the asr3-1 mutant displayed overall enhanced flg22 response whereas OX9 displayed overall reduced responses compared to WT plants.
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| Overall design |
RNA-seq analysis was performed with 10-day-old seedlings of WT, asr3-1 and 35S:ASR3-HA transgenic line OX9 with or without 100 nM flg22 treatment for 30 min. Two independent repeats were performed for RNA-seq analysis. For each repeat, equal amount of RNA from two biological replicates was pooled for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out on an Illumina HiSeq 2500 platform with 100 nt single end reads.
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| Contributor(s) |
Li B, Jiang S, Yu X, Cheng C, Chen S, Cheng Y, Yuan JS, Jiang D, He P, Shan L |
| Citation(s) |
25770109 |
| |
| Submission date |
Nov 24, 2014 |
| Last update date |
Nov 01, 2019 |
| Contact name |
Libo Shan |
| Organization name |
Texas A&M University
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| Department |
Department of Plant Pathology and Microbiology
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| Lab |
Institute for Plant Genomics and Biotechnology
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| Street address |
Norman Borlaug Center 136, TAMU2123
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| City |
College Station |
| State/province |
Texas |
| ZIP/Postal code |
77845 |
| Country |
USA |
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| Platforms (1) |
| GPL17639 |
Illumina HiSeq 2500 (Arabidopsis thaliana) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA268383 |
| SRA |
SRP050224 |
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