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Series GSE63398 Query DataSets for GSE63398
Status Public on Dec 23, 2014
Title Ets homologous factor regulates pathways controlling response to injury in Calu-3 airway epithelial cells [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Ets homologous factor (EHF) is an Ets family transcription factor expressed in many epithelial cell types including those lining the respiratory system. Disruption of the airway epithelium is central to many lung diseases, and a network of transcription factors coordinates its normal function. EHF can act as a transcriptional activator or a repressor, though its targets in lung epithelial cells are largely uncharacterized. Chromatin immunoprecipitation followed by deep sequencing (ChIP-seq), showed that the majority of EHF binding sites in lung epithelial cells are intergenic or intronic and coincide with putative enhancers, marked by specific histone modifications. EHF occupies many genomic sites that are close to genes involved in intercellular and cell-matrix adhesion.
 
Overall design Examination of EHF binding plus two histone modifications in Calu-3 cells. There are two replicates of the EHF ChIP-seq plus an input sample (control). There is one replicate each of the two histone modifications, plus a second input sample (control).
 
Contributor(s) Fossum S, Mutolo M, Yang R, Dang H, O'Neal W, Knowles M, Leir SH, Harris A
Citation(s) 25414352
Submission date Nov 18, 2014
Last update date May 15, 2019
Contact name Ann Harris
Organization name Lurie Children's Hospital of Chicago Research Center
Department Human Molecular Genetics
Street address 2430 N Halsted Street Box 211
City Chicago
State/province IL
ZIP/Postal code 60614
Country USA
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (6)
GSM1548070 Input 1
GSM1548071 EHF ChIP-seq replicate 1
GSM1548072 EHF ChIP-seq replicate 2
This SubSeries is part of SuperSeries:
GSE63400 Ets homologous factor regulates pathways controlling response to injury in Calu-3 airway epithelial cells
Relations
BioProject PRJNA267665
SRA SRP049989

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE63398_RAW.tar 1000.0 Kb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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