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Series GSE63362 Query DataSets for GSE63362
Status Public on Jan 05, 2015
Title Identification of sexually dimorphically expressed genes in rat tissues
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary The sexually dimorphic expression of genes across 26 somatic rat tissues was using Affymetrix RAE-230 genechips. We considered probesets to be sexually dimorphically expressed (SDE) if they were measurably expressed above background in at least one sex, there was at least a two-fold difference in expression (dimorphism) between the sexes, and the differences were statistically significant after correcting for false discovery. 14.5% of expressed probesets were SDE in at least one tissue, with higher expression nearly twice as prevalent in males compared to females. Most were SDE in a single tissue. Surprisingly, nearly half of the probesets that were (SDE) in multiple tissues were oppositely sex biased in different tissues, and most SDE probesets were also expressed without sex bias in other tissues. Two genes were widely SDE: Xist (female-only) and Eif2s3y (male-only). The frequency of SDE probesets varied widely between tissues, and was highest in the duodenum (6.2%), whilst less than 0.05% in over half of the surveyed tissues. The occurrence of SDE probesets was not strongly correlated between tissues. Within individual tissues, however, relational networks of SDE genes were identified. In the liver, networks relating to differential metabolism between the sexes were seen. The estrogen receptor was implicated in differential gene expression in the duodenum. To conclude, sexually dimorphic gene expression is common, but highly tissue-dependent. Sexually dimorphic gene expression may provide insights into mechanisms underlying phenotypic sex differences.
Overall design 5 male and 5 female animals used. Data provided for all files that meet QC criteria
Contributor(s) Huby R, Glaves P, Jackson R
Citation(s) 25548914
Submission date Nov 17, 2014
Last update date Jul 31, 2017
Contact name Russell Huby
Organization name AstraZeneca
Street address Alderley Park
City Macclesfield
State/province Cheshire
ZIP/Postal code SK10 4TG
Country United Kingdom
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (256)
GSM1547228 Adipose male 1
GSM1547231 Adipose male 2
GSM1547232 Adipose male 3
BioProject PRJNA267579

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE63362_RAW.tar 626.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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