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| Status |
Public on Dec 01, 2018 |
| Title |
Expression profile of Ras, MEK and ERK inhibited 16HBE bronchial epithelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To identify Ras, MEK and ERK target genes which regulate tight junction formation in 16HBE bronchial epithelial cells
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| Overall design |
16HBE cells were stably infected with pQCXIP retroviral vector, or pQCXIP expressing dominant negative Ras (DN Ras). pQCXIP cells were treated with DMSO (control), 500nM GSK1120212 (MEK inhibitor) or 1uM SCH772984 (ERK inhibitor) for 4 days, then total RNA was extracted. 4 samples were harvested per experiment: pQCXIP/DMSO, pQCXIP/MEKi, pQCXIP/ERKi, pQCXIP.DN Ras. This was repeated in 3 independent experiments. Gene expression was compared to the pQCXIP/DMSO control.
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| Contributor(s) |
Durgan J, Tao G, Walters MS, Florey O, Schmidt A, Arbelaez V, Rosen N, Crystal RG, Hall A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 12, 2014 |
| Last update date |
Dec 02, 2018 |
| Contact name |
Jeffrey Zhao |
| Organization name |
Memorial Sloan Kettering Cancer Center
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| Department |
Genomics Core
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| Street address |
1275 York Avenue
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA267018 |
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