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| Status |
Public on Nov 07, 2014 |
| Title |
Expression data from glioblastoma stem-like cells (GSCs) and astrocyte co-cultured GSCs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
consequences of astrocytes on GSCs, gene expression profiles generated from glioblastoma stem-like cells grown alone (mono-culture) and compared to those generated 48h after the initiation of co-culture with astrocytes
We used microarrays show that astrocytes are capable to modify via a paracrine mechanism GSC gene expression and thus phenotype.
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| Overall design |
GSCs were seeded into 6-wellplates with astrocytes onto the corresponding trans-well inserts. Co-cultures were initiated by combining the inserts and the 6-well plates; 48h later RNA was collected from GSCs. RNA was collected from three biological replicates was processed and subjected to microarray analysis on Human Genome U133A 2.0 chips.
NSC11: glioblastoma stem-like cells isolated from human glioblastoma surgical specimen patient 1
GBAM1: glioblastoma stem-like cells isolated from human glioblastoma surgical specimen patient 2
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| Contributor(s) |
Rath BH, Tofilon P |
| Citation(s) |
26518290 |
| Submission date |
Nov 06, 2014 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Barbara Helen Rath |
| E-mail(s) |
rathbh@mail.nih.gov
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| Organization name |
National Institute of Health
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| Department |
National Cancer Institute
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| Lab |
Radiation Oncology
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| Street address |
10 Center Drive B3B406
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA266486 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE63037_RAW.tar |
25.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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