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Series GSE62635 Query DataSets for GSE62635
Status Public on Dec 23, 2014
Title Transcriptional responses of mouse adipocytes during insulin resistance
Organism Mus musculus
Experiment type Expression profiling by array
Summary Insulin resistance is a sine qua non of Type 2 diabetes (T2D) and a frequent complication of multiple clinical conditions, including obesity, aging, and steroid use, among others. How such a panoply of insults can result in a common phenotype is incompletely understood. Furthermore, very little is known about the transcriptional and epigenetic basis of this disorder, despite evidence that such pathways are likely to play a fundamental role. Here, we compare cell autonomous models of insulin resistance induced by the cytokine tumor necrosis factor-a (TNF) or by the steroid dexamethasone (Dex) to construct detailed transcriptional profiles associated with cellular insulin resistance.
Gene expression data from mouse adipocyte, with TNF or Dex treatments at different time points.
Overall design Murine 3T3-L1 adipocytes were treated separately with dexamethasone (Dex; 20nM) or tumor necrosis factor-alpha. To comprehensively assess gene expression changes caused by Dex and TNF in a time-dependent manner, we profiled cells at early (2 hours), intermediate (24 hours), and late (6 days) points in the development of insulin resistance.
Contributor(s) Rosen E
Citation(s) 25503565
Submission date Oct 22, 2014
Last update date Feb 11, 2019
Contact name Evan Rosen
Organization name Beth Israel Deconess Medical Center
Department Endocrinology
Lab Rosen Lab
Street address 3 Blackfan Cir
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (21)
GSM1530547 CTL, biological rep1
GSM1530548 CTL, biological rep2
GSM1530549 CTL, biological rep3
BioProject PRJNA264580

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Supplementary file Size Download File type/resource
GSE62635_RAW.tar 75.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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