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Series GSE62513 Query DataSets for GSE62513
Status Public on Feb 18, 2015
Title c-Myc Transcriptionally Amplifies Sox2 Target Genes to Regulate Self-Renewal in Multipotent Otic Progenitor Cells [ChIP-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary An immortalized multipotent otic progenitor (iMOP) cell was generated by transient expression of c-Myc in Sox2-expressing otic progenitor cells. The procedure activated endogenous c-Myc expression in the cells and amplified existing Sox2-dependent transcripts to promote self-renewal. Downregulation of c-Myc expression following growth factor withdrawal resulted in a molecular switch from self-renewal to otic differentiation.
 
Overall design ChIP-Seq was accomplished by immunoprecipitating endogenous RNA PolII, c-Myc and Sox2
 
Contributor(s) Kwan K, Shen J, Corey D
Citation(s) 25497456, 26209310
Submission date Oct 20, 2014
Last update date May 15, 2019
Contact name David P Corey
E-mail(s) david_corey@hms.harvard.edu
Phone 617-432-2506
Organization name Harvard Medical School
Department Neurobiology
Street address 220 Longwood Ave.
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
Samples (7)
GSM1528103 PolII ChIP 1
GSM1528104 PolII ChIP 2
GSM1528105 cMyc ChIP 1
This SubSeries is part of SuperSeries:
GSE62514 c-Myc Transcriptionally Amplifies Sox2 Target Genes to Regulate Self-Renewal in Multipotent Otic Progenitor Cells
Relations
BioProject PRJNA264315
SRA SRP049060

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62513_RAW.tar 4.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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