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| Status |
Public on Oct 20, 2014 |
| Title |
Expression data from two sorted lymphatic endothelial cell (LEC) populations, podoplanin-high versus podoplanin low |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Extracorporeal shockwave treatment was shown to improve orthopaedic diseases, wound healing and to stimulate lymphangiogenesis in vivo. The aim of this study was to investigate in vitro shockwave treatment (IVSWT) effects on lymphatic endothelial cell (LEC) behavior and lymphangiogenesis. We analyzed migration, proliferation, vascular tube forming capability and marker expression changes of LECs after IVSWT compared with HUVECs. Finally, transcriptome- and miRNA analyses were conducted to gain deeper insight into the IVSWT-induced molecular mechanisms in LECs. The results indicate that IVSWT-mediated proliferation changes of LECs are highly energy flux density-dependent and LEC 2D as well as 3D migration was enhanced through IVSWT. IVSWT suppressed HUVEC 3D migration but enhanced vasculogenesis. Furthermore, we identified podoplaninhigh and podoplaninlow cell subpopulations, whose ratios changed upon IVSWT treatment. Transcriptome- and miRNA analyses on these populations showed differences in genes specific for signaling and vascular tissue. Our findings help to understand the cellular and molecular mechanisms underlying shockwave-induced lymphangiogenesis in vivo.
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| Overall design |
Immortalized lymphatic endothelial cells were flow-sorted in two populations according to their differences in FSC and SSC values and subjected to Affymetrix analysis in triplicate samples
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| Contributor(s) |
Rohringer S, Holnthoner W, Hackel M, Weihs AM, Ruenzler D, Skalicky S, Karbiener M, Scheideler M, Proell J, Gabriel C, Schweighofer B, Groeger M, Spittler A, Grillari J, Redl H |
| Citation(s) |
25502694, 26484194 |
| Submission date |
Oct 20, 2014 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Bernhard Schweighofer |
| E-mail(s) |
bernhard.schweighofer@meduniwien.ac.at
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| Organization name |
Medical University of Vienna
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| Department |
Department of Dermatology
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| Street address |
Lazarettgasse 14
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| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA264309 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE62510_RAW.tar |
20.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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