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Series GSE62463 Query DataSets for GSE62463
Status Public on Apr 05, 2015
Title Effects of shear stress on cells of the aorta-gonad-mesonephros (AGM)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Blood flow promotes emergence of definitive hematopoietic stem cells (HSCs) in the developing embryo, yet the signals generated by hemodynamic forces that influence hematopoietic potential remain poorly defined. In transplantation assays of hematopoietic reconstitution, we find that fluid shear stress endows long-term multilineage engraftment potential upon early hematopoietic tissues at E9.5 not previously described to harbor HSCs. Effects on hematopoiesis appear to be mediated in part by prostaglandin E2 (PGE2) and the cyclic AMP-protein kinase A (cAMP-PKA) signaling axis. Studies of Ncx1 cardiac mutants corroborate that blood flow is required for sufficient COX2 levels and phosphorylation of CREB. Further implicating PGE2 in mediating the effects of shear stress, we find that E10.5 and E11.5 AGM treated transiently with the synthetic analog dmPGE2 engraft more robustly and contribute to greater lymphoid reconstitution. These data provide a mechanism by which biomechanical forces induced by blood flow modulate hematopoietic potential.
Overall design AGM from C57BL/6J embryos at 10.5 days gestation (E10.5) were isolated by microdissection from uteri of pregnant dams, following by gentle dissociation by Accutase with agitation at room temperature for 20 minutes. Single cell suspension was seeded on microfluidic IBIDI VI^0.4 6-channel slides (0.8 to 1x10^7 cells per channel) and permitted to attach for 8 hours. Fluid movement was then applied to each channel using a Harvard Apparatus PHD ULTRA programmable syringe pump for manangement of M5300 Myelocult medium. Cells were exposed either to static/low flow (0.0001 dyne/cm^2) or wall shear stress (WSS) of 5 dyne/cm^2 for 6 hours or 36 hours. In addition, some cells were treated with 10 uM indomethacin (indo) to inhibit COX2 activity and PGE2 synthesis. Upon collection of cells with RLT lysis buffer (QIAGEN RNeasy kit), six channels of identical treatment were pooled to comprise a single sample. 24 samples total are included in this study. 12 samples were collected after 6 hours and 12 after 36 hours. In detail, samples included at 6 hours: 3 static, 3 static with indo, 3 WSS, 3 WSS with indo; and at 36 hours: 3 static, 3 static with indo, 3 WSS, 3 WSS with indo. Sample labels begin with the timepoint collected and end with the replicate number, i.e., 06WSS1 for the 6 hour collection of the first replicate of the WSS sample.
Contributor(s) Wenzel PL
Citation(s) 25870199
Submission date Oct 17, 2014
Last update date Nov 24, 2017
Contact name Pamela L Wenzel
Organization name University of Texas Medical School at Houston
Department Pediatric Surgery
Lab Pamela Wenzel
Street address 1825 Pressler St, SRB637A
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
Platforms (1)
GPL17543 Illumina MouseWG-6 v2.0 R2 expression beadchip
Samples (24)
GSM1527354 AGM static 6hrs rep1
GSM1527355 AGM static 6hrs rep2
GSM1527356 AGM static 6hrs rep3
BioProject PRJNA264187

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62463_RAW.tar 49.2 Mb (http)(custom) TAR (of IDAT)
GSE62463_non-normalized.txt.gz 4.2 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data provided as supplementary file
Raw data are available on Series record

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