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Series GSE62428 Query DataSets for GSE62428
Status Public on May 26, 2015
Title Chip-seq analysis of CHD8 distribution in T47D cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary CHD8 is an ATPase of the SNF2 family involved in ATP-dependent nucleosome remodeling. Here we report data of the occupancy of CHD8 by ChIP-seq in human breast carcinoma T47D cells under different growing conditions. We show that under normal proliferation conditions CHD8 is mostly associated with promoters. Then, cells were subjected to 48 h or serum starvation followed by stimulation with the progesterone receptor (PR) agonist R5020 or with vehicle (ethanol), for 5 and 45 minutes. R5020 induced the fast association of CHD8 with a number of intergenic or intronic regions. We found that these regions were enriched in PR binding sites. Furthermore, they were enriched in H3K4me1 and P300, typical markers of enhancers. Therefore, our data indicate that in the presence of the PR agonist CHD8 is recruited to a number of PR enhancers. In additional experiments we demonstrated that CHD8 is required for activation of PR enhancers and for transcriptional regulation of progesterone-dependent genes.
Overall design Examination of CHD8 distribution in five different growing conditions and one IgG control sample.
Contributor(s) Subtil-Rodriguez A, Reyes JC
Citation(s) 25894978
Submission date Oct 16, 2014
Last update date May 15, 2019
Contact name Jose C. Reyes
Phone 34-954467842
Organization name CABIMER
Department Molecular biology
Lab 2
Street address Av. Américo Vespucio
City Sevilla
ZIP/Postal code 41092
Country Spain
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (6)
GSM1526889 Chip-seq_CHD8_exp
GSM1526890 Chip-seq_CHD8_5_R5020
GSM1526891 Chip-seq_CHD8_5_EtOH
BioProject PRJNA264081
SRA SRP048991

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Supplementary file Size Download File type/resource
GSE62428_RAW.tar 2.3 Gb (http)(custom) TAR (of WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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