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Series GSE62310 Query DataSets for GSE62310
Status Public on Dec 10, 2014
Title RNA-SEQ analysis of the developing lower urogenital region
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We will use micro-dissection and FACS techniques to isolate cell types from the developing lower urogenital region and perform a transcriptional analysis using RNA-SEQ. This analysis will determine the transcriptional profile of each cell type, identify compartment specific transcripts, compartment specific transcript isoforms and cell-type specific long-noncoding RNAs. In addition the unbiased nature of RNA-SEQ will potentially identify novel transcripts that have not been annotated in the database.
 
Overall design Total RNA was obtained from FACS isolated embryonic day 17.5 bladders using Tg(Upk2-cre)6Xrw x Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo transgenic mice. The long term goal is to generate a transcriptional atlas of developing lower urogenital region.
Web link http://gudmap.org/gudmap/pages/ngd_series.html?seriesId=5
 
Contributor(s) Schneider K, Brunskill EW, Mendelsohn C
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Submission date Oct 14, 2014
Last update date May 15, 2019
Contact name GUDMAP Developers
E-mail(s) gudmap-db@gudmap.org
Phone +44 131 651 8500
Organization name IGMM MRC Human Genetics Unit
Lab GUDMAP Database Group
Street address Crewe Road
City Edinburgh
ZIP/Postal code EH4 2XU
Country United Kingdom
 
Platforms (1)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
Samples (10)
GSM1525107 13156_CM4G
GSM1525108 13157_CM6G
GSM1525109 E17SHH_14G
Relations
BioProject PRJNA263812
SRA SRP048901

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62310_RAW.tar 11.1 Mb (http)(custom) TAR (of FPKM_TRACKING)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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