|
Status |
Public on Dec 10, 2014 |
Title |
RNA-SEQ analysis of the developing lower urogenital region |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
We will use micro-dissection and FACS techniques to isolate cell types from the developing lower urogenital region and perform a transcriptional analysis using RNA-SEQ. This analysis will determine the transcriptional profile of each cell type, identify compartment specific transcripts, compartment specific transcript isoforms and cell-type specific long-noncoding RNAs. In addition the unbiased nature of RNA-SEQ will potentially identify novel transcripts that have not been annotated in the database.
|
|
|
Overall design |
Total RNA was obtained from FACS isolated embryonic day 17.5 bladders using Tg(Upk2-cre)6Xrw x Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo transgenic mice. The long term goal is to generate a transcriptional atlas of developing lower urogenital region.
|
Web link |
http://gudmap.org/gudmap/pages/ngd_series.html?seriesId=5
|
|
|
Contributor(s) |
Schneider K, Brunskill EW, Mendelsohn C |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
Submission date |
Oct 14, 2014 |
Last update date |
May 15, 2019 |
Contact name |
GUDMAP Developers |
E-mail(s) |
gudmap-db@gudmap.org
|
Phone |
+44 131 651 8500
|
Organization name |
IGMM MRC Human Genetics Unit
|
Lab |
GUDMAP Database Group
|
Street address |
Crewe Road
|
City |
Edinburgh |
ZIP/Postal code |
EH4 2XU |
Country |
United Kingdom |
|
|
Platforms (1) |
GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
|
Samples (10)
|
|
Relations |
BioProject |
PRJNA263812 |
SRA |
SRP048901 |