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| Status |
Public on Feb 01, 2007 |
| Title |
Expression data from rust or mock inoculated, fully expanded flag leaf halves |
| Organism |
Triticum aestivum |
| Experiment type |
Expression profiling by array
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| Summary |
Two sets of wheat lines near-isogenic to Lr34 were used to compare gene expression profiles of wheat: 1. with and without Lr34 gene; 2. rust and mock inoculation; 3. distal and basal portion of the flag leaves. The two sets of wheat near-isogenic lines were used to subtract genetic background variations and to enrich Lr34-regulated gene expression profiles. The study is aimed to better understand the mechanisms of the well-known durable leaf rust resistance gene, Lr34, mediated resistance at the transcriptome level.
Keywords: Distal and basal leaf halves of near-isogenic lines
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| Overall design |
Wheat near-isogenic lines, Jupateco with Lr34 (JUR), Jupateco without Lr34 (JUS), Thatcher with Lr34 (THR), and Thatcher without Lr34 (THS) were used. Thatcher lines were rust (I) or mock (M) inoculated. Jupateco lines were mock inoculated. Distal (T) and basal (B) half of the leaves were harvested and processed separately. Three biological replications were applied to each treatment.
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| Contributor(s) |
Hulbert SH, Bai J, Fellers JP, Bowden RL |
| Citation(s) |
18944173 |
| Submission date |
Nov 06, 2006 |
| Last update date |
Dec 04, 2018 |
| Contact name |
Jianfa Bai |
| E-mail(s) |
jbai@ksu.edu
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| Phone |
785-532-4332
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| Organization name |
Kansas State University
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| Department |
Diagnostic Medicine and Pathobiology
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| Street address |
1800 Denison Ave
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| City |
Manhattan |
| State/province |
KS |
| ZIP/Postal code |
66506-5502 |
| Country |
USA |
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| Platforms (1) |
| GPL3802 |
[wheat] Affymetrix Wheat Genome Array |
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| Samples (36)
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| GSM143490 |
Jupateco_Lr34 (resistant), mock inoculated basal leaf, replication 1 |
| GSM143491 |
Jupateco_Lr34 (resistant), mock inoculated basal leaf, replication 2 |
| GSM143492 |
Jupateco_Lr34 (resistant), mock inoculated basal leaf, replication 3 |
| GSM143493 |
Jupateco_Lr34 (resistant), mock inoculated distal leaf, replication 1 |
| GSM143494 |
Jupateco_Lr34 (resistant), mock inoculated distal leaf, replication 2 |
| GSM143495 |
Jupateco_Lr34 (resistant), mock inoculated distal leaf, replication 3 |
| GSM143496 |
Jupateco_Lr34 (susceptible), mock inoculated basal leaf, replication 1 |
| GSM143497 |
Jupateco_Lr34 (susceptible), mock inoculated basal leaf, replication 2 |
| GSM143498 |
Jupateco_Lr34 (susceptible), mock inoculated basal leaf, replication 3 |
| GSM143499 |
Jupateco_Lr34 (susceptible), mock inoculated distal leaf, replication 1 |
| GSM143500 |
Jupateco_Lr34 (susceptible), mock inoculated distal leaf, replication 2 |
| GSM143501 |
Jupateco_Lr34 (susceptible), mock inoculated distal leaf, replication 3 |
| GSM143502 |
Thatcher_Lr34 (resistant), rust inoculated basal leaf, replication 1 |
| GSM143503 |
Thatcher_Lr34 (resistant), rust inoculated basal leaf, replication 2 |
| GSM143504 |
Thatcher_Lr34 (resistant), rust inoculated basal leaf, replication 3 |
| GSM143505 |
Thatcher_Lr34 (resistant), rust inoculated distal leaf, replication 1 |
| GSM143506 |
Thatcher_Lr34 (resistant), rust inoculated distal leaf, replication 2 |
| GSM143507 |
Thatcher_Lr34 (resistant), rust inoculated distal leaf, replication 3 |
| GSM143508 |
Thatcher_Lr34 (resistant), mock inoculated basal leaf, replication 1 |
| GSM143509 |
Thatcher_Lr34 (resistant), mock inoculated basal leaf, replication 2 |
| GSM143510 |
Thatcher_Lr34 (resistant), mock inoculated basal leaf, replication 3 |
| GSM143511 |
Thatcher_Lr34 (resistant), mock inoculated distal leaf, replication 1 |
| GSM143512 |
Thatcher_Lr34 (resistant), mock inoculated distal leaf, replication 2 |
| GSM143513 |
Thatcher_Lr34 (resistant), mock inoculated distal leaf, replication 3 |
| GSM143515 |
Thatcher_Lr34 (susceptible), rust inoculated basal leaf, replication 1 |
| GSM143516 |
Thatcher_Lr34 (susceptible), rust inoculated basal leaf, replication 2 |
| GSM143517 |
Thatcher_Lr34 (susceptible), rust inoculated basal leaf, replication 3 |
| GSM143518 |
Thatcher_Lr34 (susceptible), rust inoculated distal leaf, replication 1 |
| GSM143519 |
Thatcher_Lr34 (susceptible), rust inoculated distal leaf, replication 2 |
| GSM143520 |
Thatcher_Lr34 (susceptible), rust inoculated distal leaf, replication 3 |
| GSM143521 |
Thatcher_Lr34 (susceptible), mock inoculated basal leaf, replication 1 |
| GSM143522 |
Thatcher_Lr34 (susceptible), mock inoculated basal leaf, replication 2 |
| GSM143523 |
Thatcher_Lr34 (susceptible), mock inoculated basal leaf, replication 3 |
| GSM143524 |
Thatcher_Lr34 (susceptible), mock inoculated distal leaf, replication 1 |
| GSM143525 |
Thatcher_Lr34 (susceptible), mock inoculated distal leaf, replication 2 |
| GSM143526 |
Thatcher_Lr34 (susceptible), mock inoculated distal leaf, replication 3 |
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| Relations |
| BioProject |
PRJNA97589 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE6227_RAW.tar |
171.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Raw data provided as supplementary file |
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