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Series GSE6227 Query DataSets for GSE6227
Status Public on Feb 01, 2007
Title Expression data from rust or mock inoculated, fully expanded flag leaf halves
Organism Triticum aestivum
Experiment type Expression profiling by array
Summary Two sets of wheat lines near-isogenic to Lr34 were used to compare gene expression profiles of wheat: 1. with and without Lr34 gene; 2. rust and mock inoculation; 3. distal and basal portion of the flag leaves. The two sets of wheat near-isogenic lines were used to subtract genetic background variations and to enrich Lr34-regulated gene expression profiles. The study is aimed to better understand the mechanisms of the well-known durable leaf rust resistance gene, Lr34, mediated resistance at the transcriptome level.
Keywords: Distal and basal leaf halves of near-isogenic lines
Overall design Wheat near-isogenic lines, Jupateco with Lr34 (JUR), Jupateco without Lr34 (JUS), Thatcher with Lr34 (THR), and Thatcher without Lr34 (THS) were used. Thatcher lines were rust (I) or mock (M) inoculated. Jupateco lines were mock inoculated. Distal (T) and basal (B) half of the leaves were harvested and processed separately. Three biological replications were applied to each treatment.
Contributor(s) Hulbert SH, Bai J, Fellers JP, Bowden RL
Citation(s) 18944173
Submission date Nov 06, 2006
Last update date Dec 04, 2018
Contact name Jianfa Bai
Phone 785-532-4332
Organization name Kansas State University
Department Diagnostic Medicine and Pathobiology
Street address 1800 Denison Ave
City Manhattan
State/province KS
ZIP/Postal code 66506-5502
Country USA
Platforms (1)
GPL3802 [wheat] Affymetrix Wheat Genome Array
Samples (36)
GSM143490 Jupateco_Lr34 (resistant), mock inoculated basal leaf, replication 1
GSM143491 Jupateco_Lr34 (resistant), mock inoculated basal leaf, replication 2
GSM143492 Jupateco_Lr34 (resistant), mock inoculated basal leaf, replication 3
BioProject PRJNA97589

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6227_RAW.tar 171.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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