 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 01, 2015 |
| Title |
S-adenosylmethionine (SAM) changes DNA methylation patterns in prostate cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
|
| Summary |
Genome-wide DNA methylation profiling of human PC3 invasive prostate cancer cell line treated with vehicle control (SAH, S-adenosylhomocysteine) and with SAM (S-adenosylmethionine) as well as of untreated human LNCaP non-invasive prostate cancer cell line. The Illumina Infinium 450k Human DNA Methylation BeadChip v1.2 was used to obtain DNA methylation profiles across approximately 450,000 CpGs in human cell lines exposed to described treatments. Samples included biological triplicate of PC3 control (SAH treated), biological triplicate of PC3 treated with SAM, and biological duplicate of LNCaP untreated.
|
| |
|
| Overall design |
Bisulfite-converted DNA from the 8 samples were hybridised to the Illumina Infinium 450k Human Methylation BeadChip v1.2.
|
| |
|
| Contributor(s) |
Shukeir N, Stefanska B, Parashar S, Chik F, Arakelian A, Szyf M, Rabbani SA |
| Citation(s) |
25631332 |
| Submission date |
Oct 03, 2014 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Barbara Stefanska |
| E-mail(s) |
barbara.stefanska@ubc.ca
|
| Organization name |
The University of British Columbia
|
| Department |
Land and Food Systems
|
| Lab |
Stefanska Lab
|
| Street address |
2205 East Mall
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6T 1Z4 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
|
| Samples (8)
|
| GSM1519011 |
PC3 cells treated with vehicle control (SAH), biological replicate 1 |
| GSM1519012 |
PC3 cells treated with vehicle control (SAH), biological replicate 2 |
| GSM1519013 |
PC3 cells treated with vehicle control (SAH), biological replicate 3 |
| GSM1519014 |
PC3 cells treated with SAM, biological replicate 1 |
| GSM1519015 |
PC3 cells treated with SAM, biological replicate 2 |
| GSM1519016 |
PC3 cells treated with SAM, biological replicate 3 |
| GSM1519017 |
LNCaP cells untreated, biological replicate 1 |
| GSM1519018 |
LNCaP cells untreated, biological replicate 2 |
|
| Relations |
| BioProject |
PRJNA263087 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE62053_RAW.tar |
249.4 Mb |
(http)(custom) |
TAR (of IDAT) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...