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Series GSE61993 Query DataSets for GSE61993
Status Public on Oct 03, 2014
Title Expression data from human keratinocytes stimulated with streptococcal M1 protein
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We used microarray analysis to investigate if keratinocytes excert an immuno-inflammatory response towards streptococcal M1 protein.
Keratinocytes respond to M1 protein by activating pathways to induce the production of inflammatory signaltransducers, AP-1 and NFkB transcription factor subunits, together with inflammatory mediators such as IL-8, CCL20 and IL-1 family proteins etc.
 
Overall design HaCaT cells were cultured in serum-free media, without calcium, until 80% confluency. Cells were incubated 6 h in absence or presence of M1 protein before RNA extraction. The integrity of RNA quality was analysed by an Bioanalyzer. Out of 12 samples, 3 M1 stimulated and 3 control samples were chosen for hybridization on Affymetrix Human 2.0 ST array.
 
Contributor(s) Persson S, Wilk L, Mörgelin M, Herwald H
Citation(s) 26416902
Submission date Oct 02, 2014
Last update date Dec 11, 2017
Contact name Sandra Therese Persson
E-mail(s) sandra.persson@med.lu.se
Organization name Lund University
Department Department of Clinical Sciences
Lab Division of Infection Medicine, BMC C14
Street address Tornavägen 10
City Lund
ZIP/Postal code 221 84
Country Sweden
 
Platforms (1)
GPL17692 [HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array [transcript (gene) version]
Samples (6)
GSM1518006 HaCaT_control_biological rep 1
GSM1518007 HaCaT_control_biological rep 2
GSM1518008 HaCaT_control_biological rep 3
Relations
BioProject PRJNA262904

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Supplementary file Size Download File type/resource
GSE61993_RAW.tar 50.8 Mb (http)(custom) TAR (of CEL)
GSE61993_SAM_T-test_C_vs_M_q_0_pvalue_0_0001_.txt.gz 156.4 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table

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