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Series GSE61910 Query DataSets for GSE61910
Status Public on Mar 01, 2016
Title Reduced expression of RBM47 enhances Nrf2 activity to promote tumor growth in A549 lung adenocarcinoma cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Other
Summary We identified RNA binding motif protein 47 (RBM47) as a target gene of transforming growth factor (TGF)-beta in mammary gland epithelial cells (NMuMG cells) that have undergone the epithelial-to-mesenchymal transition (EMT). TGF-beta repressed RBM47 expression in NMuMG cells and lung cancer cell lines. Expression of RBM47 correlated with good prognosis in patients with lung, breast, and gastric cancer. RBM47 suppressed the expression of cell metabolism-related genes, which were the direct targets of nuclear factor erythroid 2-related factor 2 (Nrf2; also known as NFE2L2). RBM47 bound to KEAP1 and Cullin3 mRNAs, and knockdown of RBM47 inhibited their protein expression, which led to enhanced binding of Nrf2 to target genomic regions. Knockdown of RBM47 also enhanced the expression of some Nrf2 activators, p21/CDKN1A and MafK induced by TGF-beta. Both mitochondrial respiration rates and the side population cells in lung cancer cells increased in the absence of RBM47. Our findings, together with the enhanced tumor formation and metastasis of xenografted mice by knockdown of the RBM47 expression, suggested tumor suppressive roles for RBM47 through the inhibition of Nrf2 activity.
 
Overall design Effect of shRNA for RBM47 and TGF-beta on gene expression was evaluated by RNA-seq and RBM47-bound RNAs were identified by RIP-seq in A549 cells.
 
Contributor(s) Sakurai T, Isogaya K, Sakai S, Morikawa M, Morishita Y, Ehata S, Miyazono K, Koinuma D
Citation(s) 26923328
Submission date Sep 30, 2014
Last update date May 15, 2019
Contact name Daizo Koinuma
E-mail(s) d-koinuma@umin.ac.jp
Organization name University of Tokyo
Department Pathology
Street address Hongo 7-3-1, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-0033
Country Japan
 
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (8)
GSM1517072 RNA-seq, shControl, without TGF-beta
GSM1517073 RNA-seq, shControl, TGF-beta treated
GSM1517074 RNA-seq, shRBM47, without TGF-beta
Relations
BioProject PRJNA262663
SRA SRP048532

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Supplementary file Size Download File type/resource
GSE61910_RIP-seq.genes.fpkm_tracking.gz 1.1 Mb (ftp)(http) FPKM_TRACKING
GSE61910_RNA-seq.genes.fpkm_tracking.gz 1.2 Mb (ftp)(http) FPKM_TRACKING
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Raw data are available in SRA
Processed data are available on Series record

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