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Series GSE6190 Query DataSets for GSE6190
Status Public on Jan 20, 2007
Title Temperature-dependent transcriptional response under anaerobic C and N limitations in Yeast
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary The global transcriptional response of Saccharomyces cerevisiae was investigated in low temperature chemostat cultures grown in carbon or nitrogen limitation. During steady state chemostats, the growth rates and in vivo fluxes were kept constant however the growth-limiting nutrient was significantly higher at 12oC than at 30oC and had significant effects on transcriptional responses. Growth at 12oC resulted in a rearrangement of transporters for the limiting nutrient, where hexose transporters (HXTs) and ammonium permeases (MEPs) were differentially expressed in cultures grown at 30oC in carbon and nitrogen limitations, respectively. In addition, we found repression of genes encoding proteins in reserve carbohydrates metabolism and metabolism of alternative carbon or nitrogen sources other than glucose or ammonia. However, there were also similar responses when the transcriptional response was evaluated regardless of the growth-limiting nutrient. In particular, induction of ribosome biogenesis genes emphasizes the significance of transcription and translational adaptation at low temperature. In contrast, genes encoding proteins during stress response were downregulated. This down-regulation of stress elements better known as environmental stress response (ESR) is in contradiction with previous low temperature transcriptome analyses. During continuous steady state low temperature cultivation, ESR no longer plays an integral role in S. cerevisiae’s response to temperature change. Similarly, trehalose accumulation, consistent with its gene expression, was not indispensable for growth at 12oC. This response, however, does not exclude that ESR may be required for transition phase in low temperature growth when cells are transferred from one temperature to another.
Keywords: chemostat temperature 12 degree celsuis 30 degree celsius
Overall design The global transcriptional response of Saccharomyces cerevisiae was investigated in low temperature chemostat cultures grown in carbon or nitrogen limitation at a dilution rate of 0.03h-1
Contributor(s) Tai S, Daran-Lapujade P, Walsh MC, Pronk JT, Daran J
Citation(s) 17928405
Submission date Oct 30, 2006
Last update date Jul 01, 2016
Contact name Jean-Marc Daran
Phone +31 15 278 2412
Organization name Delft University of Technology
Department Department of Biotechnology
Lab Kluyver centre for genomics of industrial organisms
Street address Julianalaan 67
City Delft
ZIP/Postal code 2628BC
Country Netherlands
Platforms (1)
GPL90 [YG_S98] Affymetrix Yeast Genome S98 Array
Samples (12)
GSM143071 12C Chemostat anaerobic C-lim 0.03h-1 (1)
GSM143073 12C C-im anaerobic chemostat 0.03h-1 (2)
GSM143076 12C anaerobic C-lim chemostat 0.03h-1 (3)
BioProject PRJNA97543

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Supplementary file Size Download File type/resource
GSE6190_RAW.tar 22.3 Mb (http)(custom) TAR (of CEL, EXP)
Raw data provided as supplementary file

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