|
| Status |
Public on Mar 13, 2015 |
| Title |
Genome-wide analysis of full-length Androgen Receptor (AR) and AR Splice Variant (ARv567es) cistromes |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Androgen Receptor (AR) variants (AR-V) drive prostate cancer (PCa) resistance to first and second-generation therapies targeting endocrine regulation of AR. To understand the sets of genomic targets of full-length AR vs. AR-Vs, we conducted genome-wide ChIP-seq using isogenic pairs of genome engineering cell lines expressing either ARv567es (R1-D567) or full-length AR (R1-AD1). Our data demonstrate that androgen-activated full-length AR and AR-Vs both bind to similar genomic targets, which are enriched for high affinity androgen response elements (AREs). Overall, this study demonstrates that AR-Vs restore the broad AR cistrome that is otherwise lost during endocrine-targeted therapy.
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| |
|
| Overall design |
ChIP-Seq of full-length Androgen Receptor (AR) or AR splice variant (ARv567es) binding to genomic DNA
|
| |
|
| Contributor(s) |
Dehm SM, Chan SC |
| Citation(s) |
25908785 |
| |
| Submission date |
Sep 29, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Scott Dehm |
| E-mail(s) |
dehm@umn.edu
|
| Organization name |
Masonic Cancer Center, University of Minnesota
|
| Street address |
Mayo Mail Code 806, 420 Delaware St SE
|
| City |
Minneapolis |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (11)
|
|
| Relations |
| BioProject |
PRJNA262518 |
| SRA |
SRP048210 |
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