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Series GSE61838 Query DataSets for GSE61838
Status Public on Mar 13, 2015
Title Genome-wide analysis of full-length Androgen Receptor (AR) and AR Splice Variant (ARv567es) cistromes
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Androgen Receptor (AR) variants (AR-V) drive prostate cancer (PCa) resistance to first and second-generation therapies targeting endocrine regulation of AR. To understand the sets of genomic targets of full-length AR vs. AR-Vs, we conducted genome-wide ChIP-seq using isogenic pairs of genome engineering cell lines expressing either ARv567es (R1-D567) or full-length AR (R1-AD1). Our data demonstrate that androgen-activated full-length AR and AR-Vs both bind to similar genomic targets, which are enriched for high affinity androgen response elements (AREs). Overall, this study demonstrates that AR-Vs restore the broad AR cistrome that is otherwise lost during endocrine-targeted therapy.
Overall design ChIP-Seq of full-length Androgen Receptor (AR) or AR splice variant (ARv567es) binding to genomic DNA
Contributor(s) Dehm SM, Chan SC
Citation(s) 25908785
Submission date Sep 29, 2014
Last update date May 15, 2019
Contact name Scott Dehm
Organization name Masonic Cancer Center, University of Minnesota
Street address Mayo Mail Code 806, 420 Delaware St SE
City Minneapolis
ZIP/Postal code 55455
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (11)
GSM1515520 R1AD1_Eth_AR_rep1
GSM1515521 R1AD1_Eth_AR_rep2
GSM1515522 R1AD1_Eth_AR_rep3
BioProject PRJNA262518
SRA SRP048210

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Supplementary file Size Download File type/resource
GSE61838_RAW.tar 1.7 Gb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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