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Series GSE61748 Query DataSets for GSE61748
Status Public on Aug 21, 2017
Title Transcriptome analysis of Foxj3 or Zbtb18 stable expressed embryonic stem (ES) cell lines
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To understand the specific mechanism by which Foxj3 and Zbtb18 control RA-induced neural directional differentiation of ESCs, total mRNAs of Foxj3-ES and Zbtb18-ES were extracted and used for RNA seq and transcriptome analyses. Compared with the control, 1331 genes were differentially expressed (P < 0.05) by twofold in Foxj3-ES (557 were underexpressed and 774 were overexpressed), and 1175 genes were differentially expressed (P < 0.05) by twofold in Zbtb18-ESCs (548 were underexpressed and 627 were overexpressed). Through Gene ontology and gene co-expression network analysis, we identified four critical genes in the neural regulatory networks: Olig1, Zic5, Erbb2, and Olig2. Our study shows that Foxj3 and Zbtb18 could trigger the gene regulatory networks of neurodevelopment by mediating the expression of Olig1, Zic5, Erbb2, and Olig2.
Overall design mRNA profiles of Foxj3 or Zbtb18 stable expressed embryonic stem cell lines by deep sequencing
Contributor(s) Wu H, Zhang Y, Lv J, Zhang Y
Citation(s) 28749472
Submission date Sep 25, 2014
Last update date May 15, 2019
Contact name Haibo Wu
Organization name Northwest A&F University
Street address 3rd, Taicheng Road
City Yangling
ZIP/Postal code 712100
Country China
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (9)
GSM1513298 Control rep1
GSM1513299 Control rep2
GSM1513300 Control rep3
BioProject PRJNA262016
SRA SRP047481

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Supplementary file Size Download File type/resource
GSE61748_All_UQRPKM.xls.gz 3.1 Mb (ftp)(http) XLS
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Processed data are available on Series record
Raw data are available in SRA

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