 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Aug 21, 2017 |
| Title |
Transcriptome analysis of Foxj3 or Zbtb18 stable expressed embryonic stem (ES) cell lines |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To understand the specific mechanism by which Foxj3 and Zbtb18 control RA-induced neural directional differentiation of ESCs, total mRNAs of Foxj3-ES and Zbtb18-ES were extracted and used for RNA seq and transcriptome analyses. Compared with the control, 1331 genes were differentially expressed (P < 0.05) by twofold in Foxj3-ES (557 were underexpressed and 774 were overexpressed), and 1175 genes were differentially expressed (P < 0.05) by twofold in Zbtb18-ESCs (548 were underexpressed and 627 were overexpressed). Through Gene ontology and gene co-expression network analysis, we identified four critical genes in the neural regulatory networks: Olig1, Zic5, Erbb2, and Olig2. Our study shows that Foxj3 and Zbtb18 could trigger the gene regulatory networks of neurodevelopment by mediating the expression of Olig1, Zic5, Erbb2, and Olig2.
|
| |
|
| Overall design |
mRNA profiles of Foxj3 or Zbtb18 stable expressed embryonic stem cell lines by deep sequencing
|
| |
|
| Contributor(s) |
Wu H, Zhang Y, Lv J, Zhang Y |
| Citation(s) |
28749472 |
| |
| Submission date |
Sep 25, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Haibo Wu |
| E-mail(s) |
hbwu029@nwsuaf.edu.cn
|
| Organization name |
Northwest A&F University
|
| Street address |
3rd, Taicheng Road
|
| City |
Yangling |
| ZIP/Postal code |
712100 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA262016 |
| SRA |
SRP047481 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE61748_All_UQRPKM.xls.gz |
3.1 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...