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| Status |
Public on Sep 24, 2014 |
| Title |
Transcription profile of Δphm4 strain during growth in no phosphate medium |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Depletion of essential nutrients triggers regulatory programs that prolong cell growth and survival. Starvation-induced processes increase nutrient transport, mobilize nutrient storage, and recycle nutrients between cellular components. This leads to an effective increase in intracellular nutrients, which may act as a negative feedback that down-regulates the starvation program. To examine how cells overcome this potential instability, we followed the transcription response of budding yeast transferred to medium lacking phosphate. Genes were induced in two temporal waves. The first wave was stably maintained and persisted even upon phosphate replenishment, indicating a positive feedback loop. This commitment was abolished after two hours with the induction of the second expression wave, coinciding with the reduction in cell growth rate. We identify genes that mediate this loss of commitment, and show that the overall temporal stability of the expression response depends on the sequential pattern of gene induction. Our results emphasize the key role of gene expression dynamics in optimizing cellular adaptation.
Δphm4 cells were grown at high Phosphate medium, washed and transferred to no phosphate medium. Sample were taken every 15 minuets for 3.75 hours
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| Overall design |
16 samples were taken during the time course. Expression data was normalized to the first time point (cells grown at high phosphate medium)
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| Contributor(s) |
Vardi N |
| Citation(s) |
25437565 |
| Submission date |
Sep 23, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Noam Vardi |
| E-mail(s) |
noamvardi@gmail.com
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| Organization name |
Weizmann Institute of Science
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| Department |
molecular genetics
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| Lab |
Naama Barkai
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| Street address |
herzel 235
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| City |
rehovot |
| ZIP/Postal code |
7632700 |
| Country |
Israel |
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| Platforms (1) |
| GPL17342 |
Illumina HiSeq 2500 (Saccharomyces cerevisiae) |
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| Samples (16)
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| GSM1510526 |
phm4_del, no Pi, 45 min |
| GSM1510527 |
phm4_del, no Pi, 60 min |
| GSM1510528 |
phm4_del, no Pi, 75 min |
| GSM1510529 |
phm4_del, no Pi, 90 min |
| GSM1510530 |
phm4_del, no Pi, 105 min |
| GSM1510531 |
phm4_del, no Pi, 120 min |
| GSM1510532 |
phm4_del, no Pi, 135 min |
| GSM1510533 |
phm4_del, no Pi, 150 min |
| GSM1510534 |
phm4_del, no Pi, 165 min |
| GSM1510535 |
phm4_del, no Pi, 180 min |
| GSM1510536 |
phm4_del, no Pi, 195 min |
| GSM1510537 |
phm4_del, no Pi, 210 min |
| GSM1510538 |
phm4_del, no Pi, 225 min |
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| This SubSeries is part of SuperSeries: |
| GSE61668 |
Sequential feedback induction stabilizes the phosphate starvation response in budding yeast |
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| Relations |
| BioProject |
PRJNA261797 |
| SRA |
SRP047417 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE61667_data_matrix_phm4_del.txt.gz |
337.0 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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