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Series GSE61520 Query DataSets for GSE61520
Status Public on Sep 19, 2014
Title Mycoplasma gallisepticum Lipid Associated Membrane Proteins Up-regulate Inflammatory Genes in Chicken Tracheal Epithelial Cells via TLR-2 Ligation Through an NF-κB Dependent Pathway
Organism Gallus gallus
Experiment type Expression profiling by array
Summary To identify differential gene expression profiles of chicken tracheal epithelial cells (TECs) upon exposure to Mycoplasma gallisepticum virulent strain Rlow and avirulent strain Rhigh and corresponding lipid associated membrane proteins(LAMP) at 1.5 hours in vitro. Goal of this experiment was to identify relative comtribution of LAMPs in up-regulation of inflammatory gene compared to the live strains. Several genes were identified to be differentially regulated in all exposures, but the virulent strain up-regulated more number genes as well as at a higher extent. We identified 6 important inflammatory mediators and did confirmatory RT-qPCR analysis at 1.5, 6 and 24 hours in vitro as well as at 1.5 and 6 hours ex-vivo. RT-qPCR was also employed to identify expression of these 6 genes in presence of different signalling inhibitors and we were able to identify that Mycoplasma gallisepticum LAMPs up-regulate these inflammatory genes via TLR-2 in an NF-κB dependent pathway.
 
Overall design Primary chicken tracheal epithelial cells (TECs) were exposed to either 500 MOI of a virulent Mycoplasma gallisepticum strain Rlow or an avirulent strain Rhigh and the corresponding lipid associated membrane proteins (LAMPs) at 5µg/mL for 1.5 hours. 4 biological replicates along with a dye swap technique totalling 8 replicates were utilized for all microarray experiments
 
Contributor(s) Majumder S, Zappulla F
Citation(s) 25401327
Submission date Sep 18, 2014
Last update date Dec 19, 2014
Contact name Frank Zappulla
E-mail(s) frank.zappulla@engr.uconn.edu
Organization name University of Connecticut
Department Pathobiology
Street address 61 North Eagleville Road
City Storrs
State/province CT
ZIP/Postal code 06269
Country USA
 
Platforms (1)
GPL15357 Agilent-026441 Gallus gallus (Chicken) Oligo Microarray v2 (Probe Name version)
Samples (64)
GSM1507335 TEC-LAMP R(low) replicate 1_Cy5
GSM1507336 TEC-Media control biological replicate 1 technical replicate 1_Cy3
GSM1507337 TEC-LAMP R(low) replicate 2_Cy5
Relations
BioProject PRJNA261378

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE61520_R_high_1-1.gpr.gz 5.7 Mb (ftp)(http) GPR
GSE61520_R_high_1-2.gpr.gz 5.7 Mb (ftp)(http) GPR
GSE61520_R_high_1-3.gpr.gz 5.7 Mb (ftp)(http) GPR
GSE61520_R_high_1-4.gpr.gz 5.7 Mb (ftp)(http) GPR
GSE61520_R_high_2-1.gpr.gz 5.8 Mb (ftp)(http) GPR
GSE61520_R_high_2-2.gpr.gz 5.7 Mb (ftp)(http) GPR
GSE61520_R_high_2-3.gpr.gz 5.7 Mb (ftp)(http) GPR
GSE61520_R_high_2-4.gpr.gz 5.6 Mb (ftp)(http) GPR
GSE61520_R_high_3-1.gpr.gz 5.8 Mb (ftp)(http) GPR
GSE61520_R_high_3-2.gpr.gz 5.7 Mb (ftp)(http) GPR
GSE61520_R_high_3-3.gpr.gz 5.7 Mb (ftp)(http) GPR
GSE61520_R_high_3-4.gpr.gz 5.7 Mb (ftp)(http) GPR
GSE61520_R_high_4-1.gpr.gz 5.9 Mb (ftp)(http) GPR
GSE61520_R_high_4-2.gpr.gz 5.8 Mb (ftp)(http) GPR
GSE61520_R_high_4-3.gpr.gz 5.8 Mb (ftp)(http) GPR
GSE61520_R_high_4-4.gpr.gz 5.8 Mb (ftp)(http) GPR
GSE61520_R_low_1-1.gpr.gz 5.5 Mb (ftp)(http) GPR
GSE61520_R_low_1-2.gpr.gz 5.4 Mb (ftp)(http) GPR
GSE61520_R_low_1-3.gpr.gz 5.4 Mb (ftp)(http) GPR
GSE61520_R_low_1-4.gpr.gz 5.4 Mb (ftp)(http) GPR
GSE61520_R_low_2-1.gpr.gz 5.5 Mb (ftp)(http) GPR
GSE61520_R_low_2-2.gpr.gz 5.6 Mb (ftp)(http) GPR
GSE61520_R_low_2-3.gpr.gz 5.5 Mb (ftp)(http) GPR
GSE61520_R_low_2-4.gpr.gz 5.4 Mb (ftp)(http) GPR
GSE61520_R_low_3-1.gpr.gz 5.6 Mb (ftp)(http) GPR
GSE61520_R_low_3-2.gpr.gz 5.6 Mb (ftp)(http) GPR
GSE61520_R_low_3-3.gpr.gz 5.5 Mb (ftp)(http) GPR
GSE61520_R_low_3-4.gpr.gz 5.5 Mb (ftp)(http) GPR
GSE61520_R_low_4-1.gpr.gz 5.5 Mb (ftp)(http) GPR
GSE61520_R_low_4-2.gpr.gz 5.5 Mb (ftp)(http) GPR
GSE61520_R_low_4-3.gpr.gz 5.5 Mb (ftp)(http) GPR
GSE61520_R_low_4-4.gpr.gz 5.5 Mb (ftp)(http) GPR
Processed data included within Sample table
Raw data are available on Series record

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