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| Status |
Public on Mar 19, 2015 |
| Title |
Pioneer factors govern super-enhancer dynamics in stem cell plasticity and lineage choice |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Mouse hair follicles undergo synchronized cycles. Cyclical regeneration and hair growth is fueled by hair follicle stem cells (HFSCs) and transit-amplifying cells (TACs). We used ChIP-seq to unfold genome-wide chromatin landscapes of H3K27ac and Med1 to identify super-enhancers and dissect their biological relevance in cell identity and plasticity of HFSCs in vivo and in vitro.
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| Overall design |
Telogen quiescent hair follicle stem cells (HFSCs) and transit-amplifying cells (TACs) were FACS-purified for ChIP-sequcencing. Furthermore, cultured HFSCs were used for ChIP-sequencing.
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| Contributor(s) |
Adam RC, Fuchs E |
| Citation(s) |
25799994 |
| |
| Submission date |
Sep 10, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Rene Christian Adam |
| Organization name |
Regeneron Pharmaceuticals
|
| Street address |
777 Old Saw Mill River Road
|
| City |
Tarrytown |
| State/province |
New York |
| ZIP/Postal code |
10591 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA260731 |
| SRA |
SRP047018 |
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