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| Status |
Public on Jul 01, 2007 |
| Title |
JAM-A Knockdown in HepG2 Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Hepatocytes are polarized epithelial cells whose function depends upon their ability to distinguish between the apical and basolateral surfaces that are located at intercellular tight junctions. It has been proposed that the signaling cascades that originate at these junctions influence cellular activity by controlling gene expression in the cell nucleus. To assess the validity of this proposal with regard to hepatocytes, we depleted expression of the tight junction protein junctional adhesion molecule-A (JAM-A) in the HepG2 human hepatocellular carcinoma cell line. Reduction of JAM-A resulted in a striking change in cell morphology, with cells forming single-layered sheets instead of the normal multi-layered clusters. In the absence of JAM-A, other tight junction proteins were mislocalized, and canaliculi, which form the apical face of the hepatocyte, were consequently absent. While most changes in gene expression were modest, there was a strong transcriptional induction of the adherens junction protein E-cadherin in cells with reduced levels of JAM-A. This increase in E-cadherin was partially responsible for the observed alterations in cell morphology and mislocalization of tight junction proteins. We therefore propose that we have uncovered a novel mechanism for crosstalk between specific components of tight and adherens junctions that can be utilized to regulate adhesion between hepatic cells and to maintain hepatocyte cell polarity.
Keywords: HepG2 cells
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| Overall design |
HepG2 cells were transduced with lentiviruses and stably selected using puromycin. Comparison of four controls and five JAM-A knockdown samples.
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| Contributor(s) |
Konopka G, Duncan S |
| Citation(s) |
17623668 |
| |
| Submission date |
Oct 18, 2006 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Stephen A Duncan |
| E-mail(s) |
duncanst@musc.edu
|
| Phone |
843-792-9104
|
| Organization name |
Medical University South Carolina
|
| Department |
Regenerative Medicine and Cell Biology
|
| Lab |
Duncan Lab
|
| Street address |
173 Ashley Avenue, BSB 6th Floor, Room 657A
|
| City |
Charleston |
| State/province |
SC |
| ZIP/Postal code |
29425 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA97565 |
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