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Series GSE6065 Query DataSets for GSE6065
Status Public on Oct 21, 2006
Title Murine host cell response to Aeromonas infection
Organism Mus musculus
Experiment type Expression profiling by array
Summary Aims: To assess the virulence of multiple Aeromonas spp. using two models, a neonatal mouse assay and a mouse intestinal cell culture.
Methods and Results: Transcriptional responses to both infection models were evaluated using microarrays. After artificial infection with a variety of Aeromonas spp., mRNA extracts from the two models were processed and hydridized to murine microarrays to determine host gene response. Definition of virulence was determined based on host mRNA production in murine neonatal intestinal tissue and mortality of infected animals. Infections of mouse intestinal cell cultures were then performed to determine whether this simpler model system's mRNA responses correlated to neonatal results and therefore be predictive of virulence of Aeromonas spp. Virulent aeromonads up-regulated transcripts in both models including multiple host defense gene products (chemokines, regulation of transcription and apoptosis, cell signaling). Avirulent species exhibited little or no host response in neonates. Mortality results correlated well with both bacterial dose and average fold change of up-regulated transcripts in the neonatal mice.
Conclusions: Cell culture results were less discriminating but showed promise as potentially being able to be predictive of virulence. Jun oncogene up-regulation in murine cell culture is potentially predictive of Aeromonas virulence.
Significance and Impact of the Study: Having the ability to determine virulence of waterborne pathogens quickly would potentially assist public health officials to rapidly assess exposure risks.
Keywords: Aeromonas; Virulence; Gene expression; Host response
 
Overall design Two infection models were assessed, live, whole animals (neonatal Swiss Webster mice) and a murine small intestinal cell culture. Biological replicates (n=5) were infected with different Aeromonas species/strains and compared to uninfected controls.
 
Contributor(s) Hayes SL, Vesper SJ, Rodgers MR, Lye DJ, Stelma GN
Citation(s) 17897183
Submission date Oct 18, 2006
Last update date Feb 11, 2019
Contact name Samuel Hayes
E-mail hayes.sam@epa.gov
Phone 513-569-7514
Organization name United States Environmental Protection Agency
Department MS-387
Lab NRMRL/WSWRD
Street address 26 W. Martin Luther King Dr.
City Cincinnati
State/province OH
ZIP/Postal code 45268
Country USA
 
Platforms (3)
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
GPL340 [MOE430B] Affymetrix Mouse Expression 430B Array
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (268)
GSM139676 NRMRL control replicate01, batch032405
GSM139683 NRMRL replicate control02, batch032405
GSM139684 NRMRL control replicate03, batch032405
Relations
BioProject PRJNA97749

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6065_RAW.tar 1.2 Gb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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