|
| Status |
Public on May 06, 2015 |
| Title |
An alternative pluripotent state confers interspecies chimaeric competency [RNA-Seq] |
| Organisms |
Macaca mulatta; Pan troglodytes; Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Here we show that by simple modulation of extrinsic signaling pathways, a new class of pluripotent stem cells, referred to as region selective epiblast stem cells (rsEpiSCs), could be efficiently derived from different stages of the early embryo. rsEpiSCs share features of primed pluripotency yet are distinct from EpiSCs in their molecular characteristics and ability to colonize post-implantation embryos. We performed RNA-sequencing experiments and examined the global gene expression profiles of EpiSCs, rsEpiSCs, in vivo isolated four regions of E6.5 mouse epiblasts: AP (anterior-proximal), AD (anterior-distal), PP (posterior-proximal) and PD (posterior-distal), human H1 ESCs, H1 rsESCs, H9 ESCs, H9 rsESCs, rhesus monkey ORMES23 rsESCs, and chimpanzee rsiPSCs.
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| |
|
| Overall design |
Examination of global gene expression profiles in 2 pluripotent stem cell types across multiple species.
|
| |
|
| Contributor(s) |
Wu J, Benner C |
| Citation(s) |
25945737 |
| |
| Submission date |
Aug 21, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Christopher Benner |
| E-mail(s) |
cbenner@ucsd.edu
|
| Organization name |
University of California, San Diego (UCSD)
|
| Department |
Medicine
|
| Street address |
9500 Gilman Dr. MC 0640
|
| City |
La Jolla |
| State/province |
California |
| ZIP/Postal code |
92093-0640 |
| Country |
USA |
| |
|
| Platforms (4)
|
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19129 |
Illumina HiSeq 2500 (Macaca mulatta) |
| GPL19148 |
Illumina HiSeq 2500 (Pan troglodytes) |
|
| Samples (14)
|
|
| This SubSeries is part of SuperSeries: |
| GSE60605 |
An alternative pluripotent state confers interspecies chimaeric competency |
|
| Relations |
| BioProject |
PRJNA258641 |
| SRA |
SRP045673 |
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