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Series GSE60477 Query DataSets for GSE60477
Status Public on Jun 23, 2015
Title The oncofusion protein FUS-ERG targets key hematopoietic regulators and modulates the all-trans retinoic acid signaling pathway in t(16;21) acute myeloid leukemia
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Fusion proteins involving the ETS factor ERG have been associated with multiple cancers such as Ewing's sarcoma and prostate cancer. In acute myeloid leukemias harboring t(16;21) another ERG fusion protein is expressed, FUS-ERG. Here, we found that this FUS-ERG oncofusion protein acts in the context of a heptad of proteins (ERG, FLI1, GATA2, LYL1, LNMO2, RUNX1 and TAL1) central to proper expression of genes involved in maintaining a stem cell hematopoietic phenotype. Moreover, in t(16;21) FUS-ERG co-occupies genomic regions bound by the nuclear receptor heterodimer RXR-RARA inhibiting target gene expression and interfering with hematopoietic differentiation. All-Trans Retinoic Acid treatment of t(16;21) cells as well as FUS-ERG knock down alleviate the myeloid differentiation block. Together, the results suggest that FUS-ERG acts as a transcriptional repressor of the retinoic acid signaling pathway.
Overall design Cell lines were used for RNA extraction for RNA-seq and ChIP experiments for ChIP-seq.
Contributor(s) Sotoca AM, Prange K, Reijnders B, Mandoli A, Nguyen LN, Stunnenberg HG, Martens JH
Citation(s) 26148230
Submission date Aug 18, 2014
Last update date May 15, 2019
Contact name Joost Martens
Organization name Radboud University
Department Molecular Biology
Street address Geert Grooteplein 26
City Nijmegen
ZIP/Postal code 6525 GA
Country Netherlands
Platforms (2)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (20)
GSM1480735 ChIP ERG 1/2/3
GSM1480736 ChIP ERG 1/2/3 ATRA
GSM1480737 ChIP CTD of RNA polymerase II
BioProject PRJNA258377
SRA SRP045565

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Supplementary file Size Download File type/resource
GSE60477_RAW.tar 618.1 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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