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Series GSE60361 Query DataSets for GSE60361
Status Public on Feb 20, 2015
Title Single-cell RNA-seq of mouse cerebral cortex
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We have applied a recently developed, highly accurate and sensitive single-cell RNA-seq method (STRT/C1) to perform a molecular census of two regions of the mouse cerebral cortex: the somatosensory cortex and hippocampus CA1.
Overall design We isolated cells fresh from somatosensory cortex (S1) and hippocampus CA1 area of juvenile (P22 - P32) CD1 mice, 33 males and 34 females. Cells were collected without selection, except that 116 cells were obtained by FACS from 5HT3a-BACEGFP transgenic mice. A total of 76 Fluidigm C1 runs were performed, each attempting 96 cell captures and resulting in 3005 high-quality single-cell cDNAs, containing Unique Molecular Identifiers allowing counting of individual mRNA molecules, even after PCR amplification.
Web link
Contributor(s) Zeisel A, Muñoz Manchado AB, Lönnerberg P, Linnarsson S
Citation(s) 25700174, 28729663
Submission date Aug 12, 2014
Last update date Jun 05, 2019
Contact name Sten Linnarsson
Organization name Karolinska Institutet
Department Medical Biochemistry and Biophysics
Lab Molecular Neurobiology
Street address Scheeles väg 1
City Stockholm
ZIP/Postal code 171 65
Country Sweden
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (3005)
GSM1474251 SingleCellNo1
GSM1474252 SingleCellNo2
GSM1474253 SingleCellNo3
BioProject PRJNA258094
SRA SRP045452

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60361_C1-3005-Expression.txt.gz 10.7 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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