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| Status |
Public on Feb 20, 2015 |
| Title |
Single-cell RNA-seq of mouse cerebral cortex |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We have applied a recently developed, highly accurate and sensitive single-cell RNA-seq method (STRT/C1) to perform a molecular census of two regions of the mouse cerebral cortex: the somatosensory cortex and hippocampus CA1.
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| Overall design |
We isolated cells fresh from somatosensory cortex (S1) and hippocampus CA1 area of juvenile (P22 - P32) CD1 mice, 33 males and 34 females. Cells were collected without selection, except that 116 cells were obtained by FACS from 5HT3a-BACEGFP transgenic mice. A total of 76 Fluidigm C1 runs were performed, each attempting 96 cell captures and resulting in 3005 high-quality single-cell cDNAs, containing Unique Molecular Identifiers allowing counting of individual mRNA molecules, even after PCR amplification.
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| Web link |
http://linnarssonlab.org/cortex/
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| Contributor(s) |
Zeisel A, Muñoz Manchado AB, Lönnerberg P, Linnarsson S |
| Citation(s) |
25700174, 28729663 |
| |
| Submission date |
Aug 12, 2014 |
| Last update date |
Feb 06, 2020 |
| Contact name |
Sten Linnarsson |
| Organization name |
Karolinska Institutet
|
| Department |
Medical Biochemistry and Biophysics
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| Lab |
Molecular Neurobiology
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| Street address |
Scheeles väg 1
|
| City |
Stockholm |
| ZIP/Postal code |
171 65 |
| Country |
Sweden |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (3005)
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| Relations |
| BioProject |
PRJNA258094 |
| SRA |
SRP045452 |
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