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| Status |
Public on Feb 20, 2015 |
| Title |
Inhibition of Pseudomonas aeruginosa swarming motility by 1-naphthol and other bicyclic compounds bearing hydroxyl group. |
| Organism |
Pseudomonas aeruginosa PAO1 |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
Many bacteria convert bicyclic compounds, such as indole and naphthalene, to oxidized compounds, including hydroxyindoles and naphthols. Pseudomonas aeruginosa, a ubiquitous bacterium that inhabits diverse environments, shows pathogenicity against animals, plants and other microorganisms, and increasing evidence has shown that several bicyclic compounds alter the virulence-related phenotypes of P. aeruginosa. Here, we revealed that hydroxyindoles (4- and 5-hydroxyindoles) and naphthalene derivatives bearing hydroxyl groups specifically inhibit swarming motility but have minor effects on other motilities, including swimming and twitching, in P. aeruginosa. Further analyses using 1-naphthol showed that this effect is also associated with clinically isolated hyper swarming P. aeruginosa cells. Swarming motility is associated with the dispersion of cells from biofilms, and the addition of 1-naphthol maintained biofilm biomass without cell dispersion. Swarming inhibition did not mediate rhamnolipid production, which regulates swarming motility in P. aeruginosa. Transcriptome analyses revealed that 1-naphthol increases gene expression associated with multidrug efflux and represses gene expression associated with aerotaxis and pyochelin, flagellar, and pili synthesis. In the present study, we showed that several bicyclic compounds bearing hydroxyl groups inhibit the swarming motility of P. aeruginosa, and these results provide new insight into the chemical structures that inhibit the specific phenotypes of P. aeruginosa.
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| Overall design |
In total 4 samples: gene expressions of P. aeruginosa with (2 samples) or without (2 samples) 1-naphthol
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| Contributor(s) |
Oura H, Tashiro Y, Toyofuku M, Ueda K, Kiyokawa T, Ito S, Takahashi Y, Lee S, Nojiri H, Nakajima-Kambe T, Uchiyama H, Futamata H, Nomura N |
| Citation(s) |
25681177 |
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| Submission date |
Aug 12, 2014 |
| Last update date |
May 07, 2015 |
| Contact name |
Yosuke Tashiro |
| Organization name |
Shizuoka University
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| Department |
Graduate School of Engineering, Department of Applied Chemistry and Biochemical Engineering
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| Street address |
3-5-1 Johoku Naka-ku
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| City |
Hamamatsu |
| State/province |
Shizuoka |
| ZIP/Postal code |
433-8125 |
| Country |
Japan |
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| Platforms (1) |
| GPL8297 |
Pseudomonas aeruginosa PAO1 chromosome array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA258070 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE60343_RAW.tar |
90.5 Mb |
(http)(custom) |
TAR (of BAR, CEL) |
| Processed data provided as supplementary file |
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